A simple implantation method for flexible, multisite microelectrodes into rat brains.
A simple implantation method for flexible, multisite microelectrodes into rat brains.
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DOI：
10.3389/fneng.2013.00006
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hofmann UG
中科院分区：
文献类型：
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作者：
Richter A;Xie Y;Schumacher A;Löffler S;Kirch RD;Al-Hasani J;Rapoport DH;Kruse C;Moser A;Tronnier V;Danner S;Hofmann UG
神经组织与植入微电极之间的长期功能和可靠耦合是获取神经电生理信号或治疗性刺激神经组织的关键问题。目前经常使用的刚性微电极被认为会引起严重的异物反应，导致厚的神经胶质瘢痕，从而导致慢性期组织-电极耦合不良。我们假设，这种不良反应可能通过顺应软脑组织的探针来补救，即，用柔性电极代替刚性电极。不幸的是，这种灵活性是以低刚度为代价的，这使得靶向低创伤植入非常具有挑战性。在这项研究中，我们展示了一种适应性强且简单的方法，可以将极其灵活的微探针植入大鼠大脑的深层区域。通过暴露颅骨上的水凝胶（PBS中的2%琼脂糖）垫促进的杆支撑立体定向插入来实现柔性探针的植入。因此，我们能够在70只大鼠体内植入非常灵活的微型探针，深度达到啮齿动物的丘脑底核。这项工作详细描述了微创，但可靠的柔性探头植入所需的程序和步骤。
A long term functional and reliable coupling between neural tissue and implanted microelectrodes is the key issue in acquiring neural electrophysiological signals or therapeutically excite neural tissue. The currently often used rigid micro-electrodes are thought to cause a severe foreign body reaction resulting in a thick glial scar and consequently a poor tissue-electrode coupling in the chronic phase. We hypothesize, that this adverse effect might be remedied by probes compliant to the soft brain tissue, i.e., replacing rigid electrodes by flexible ones. Unfortunately, this flexibility comes at the price of a low stiffness, which makes targeted low trauma implantation very challenging. In this study, we demonstrate an adaptable and simple method to implant extremely flexible microprobes even to deep areas of rat's brain. Implantation of flexible probes is achieved by rod supported stereotactic insertion fostered by a hydrogel (2% agarose in PBS) cushion on the exposed skull. We were thus able to implant very flexible micro-probes in 70 rats as deep as the rodent's subthalamic nucleus. This work describes in detail the procedures and steps needed for minimal invasive, but reliable implantation of flexible probes.