Relationship Between mRNA of Immune Factors Expressed by Milk Somatic Cells and Bacteria Present in Healthy Lactating Holstein Cows
Relationship Between mRNA of Immune Factors Expressed by Milk Somatic Cells and Bacteria Present in Healthy Lactating Holstein Cows
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健康泌乳荷斯坦奶牛乳体细胞表达的免疫因子 mRNA 与细菌的关系
DOI：
10.2478/jvetres-2019-0042
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发表时间：
2019
影响因子：
1.8
通讯作者：
M. Tajima
中科院分区：
文献类型：
--
作者：
H. Ohtsuka;H. Hirose;K. Murakami;R. Murata;Toshihide Kato;M. Tajima
摘要引言泌乳奶牛体细胞中免疫因子的特征及其与正常牛奶中细菌的关系尚未阐明。本研究旨在探讨健康泌乳奶牛乳中致病菌与体细胞免疫因子的关系。材料与方法在一个奶牛场共44头健康荷斯坦奶牛进行研究。用套管无菌采集乳汁样品，并对这些样品进行培养以检测细菌和分析体细胞表达的免疫因子的mRNA。根据牛奶样品的微生物状态将奶牛分为两组：12头奶牛在培养物中显示细菌（阳性组），其他32头奶牛没有（阴性组）。结果两组奶牛体细胞IL-6、乳转铁蛋白和cathelicidin的mRNA表达水平在挤奶后均较挤奶前显著降低（P < 0.05）。挤奶前，阳性组的IL-6和cathelicidin的mRNA水平显著低于阴性组。结论健康泌乳奶牛体细胞IL-6和cathelicidin的mRNA表达水平可能受到细菌感染的影响。
Abstract Introduction The characteristics of immune factors in somatic cells from lactating dairy cows and their association with commensal bacteria in normal milk have not been clarified. This study investigated the relationship between the pathogenic bacteria in milk and somatic cell immune factors in healthy lactating cows. Material and Methods In total 44 healthy Holstein cows were studied on one farm. Milk samples were collected aseptically using a cannula and these samples were cultured for detection of bacteria and analysis of mRNA of immune factors expressed by somatic cells. Cows were divided into two groups based on the microbial status of their milk samples: 12 cows showed bacteria in cultures (positive group), and the other 32 cows did not (negative group). Results The mRNA levels of IL-6, lactotransferrin, and cathelicidin expressed by somatic cells after milking decreased significantly compared to those before milking in both groups (P < 0.05). There were significantly lower mRNA levels of IL-6 and cathelicidin in the positive group compared to those in the negative group before milking. Conclusion These results suggest that mRNA levels of IL-6 and cathelicidin expressed by the somatic cells may be affected by the presence of bacteria in healthy lactating dairy cows.