Roles of degree of fat deposition and its localization on VEGF expression in adipocytes
Roles of degree of fat deposition and its localization on VEGF expression in adipocytes
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DOI：
10.1152/ajpendo.00003.2004
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发表时间：
2005-06-01
影响因子：
5.1
通讯作者：
Saito, Y
中科院分区：
文献类型：
--
作者：
Miyazawa-Hoshimoto, S;Takahashi, K;Saito, Y
血管内皮生长因子（VEGF）是一种重要的血管生成因子，在多种细胞类型中表达。在这项研究中，我们研究了三组实验中脂肪细胞中VEGF的产生机制。首先，为了阐明血浆VEGF浓度与其在脂肪组织中表达之间的关系，我们研究了遗传性肥胖db/db和KK-A（y）小鼠。肥胖小鼠血浆VEGF浓度显著高于对照组，且与肥胖有关。内脏脂肪中VEGF的表达随生长而增强，并与脂肪沉积有关。接下来，为了证明脂肪细胞中VEGF产生和脂质积累之间的关系，我们分析了3 T3-L1细胞中VEGF mRNA表达及其蛋白分泌。在脂肪细胞转化后的3 T3-L1细胞中，在脂质积累期间VEGF的产生增强。接下来，为了阐明解剖定位对脂肪细胞中VEGF表达的作用，我们将3 T3-L1细胞植入无胸腺小鼠的内脏或皮下脂肪中。3 T3-L1细胞移植到肠系膜区后VEGF mRNA表达明显高于皮下区。植入内脏脂肪的小鼠血浆VEGF浓度高于对照组。这些结果表明，解剖定位和脂质积累是重要的VEGF在脂肪细胞中的生产。
Vascular endothelial growth factor ( VEGF) is an important angiogenic factor and is expressed in wide variety of cell types. In this study, we investigated the mechanism of VEGF production in adipocytes in three sets of experiments. First, to clarify the relation between plasma VEGF concentrations and their expressions in adipose tissues, we investigated the genetically obese db/db and KK-A(y) mice. Plasma VEGF concentrations in obese mice were significantly higher than in control and were related to adiposity. VEGF expressions in visceral fat were enhanced during growth and were related to fat deposition. Next, to demonstrate the relation between VEGF production and lipid accumulation in adipocytes, we analyzed VEGF mRNA expression and its protein secretion in 3T3-L1 cells. VEGF production was enhanced during lipid accumulation in 3T3-L1 cells after adipocyte conversion. Next, to clarify the role of anatomic localization on VEGF expression in adipocytes, we implanted 3T3-L1 cells into visceral or subcutaneous fat in athymic mice. 3T3-L1 cells implanted into the mesenteric area expressed more VEGF mRNA than that into the subcutaneous area. Plasma VEGF concentration in the mice implanted in visceral fat was higher than in controls. These results suggest that both the anatomic localization and the lipid accumulation are important for the VEGF production in adipocytes.