Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes
Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes
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DOI：
10.1128/jcm.35.7.1671-1674.1997
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发表时间：
1997-07-01
影响因子：
9.4
通讯作者：
MartinezMurcia, AJ
中科院分区：
文献类型：
--
作者：
Borrell, N;Acinas, SG;MartinezMurcia, AJ
气单胞菌属是人类的紧急病原体，由于其表型和基因组的异质性，其鉴定长期以来一直存在争议。对已发表的16S rRNA基因序列的计算机分析表明，PCR扩增的16S rRNA基因的限制性片段长度多态性是评估所有已知气单胞菌属物种身份的一种良好而快速的方法。该方法进行了评估与所有物种（或DNA同源组）的参考菌株和76个不同来源的临床分离株。两种方法的大多数结果是一致的，但也发现了一些差异。比以前的表型和遗传方法的优点进行了讨论。
Identification of Aeromonas species, emergent pathogens for humans, has long been controversial due to their phenotypic and genomic heterogeneities. Computer analysis of the published 16S rRNA gene sequences revealed that restriction fragment length polymorphism of the PCR-amplified 16S rRNA gene is a good and rapid way of assessing the identities of all known species of Aeromonas. The method was evaluated with the reference strains of all species (or DNA homology groups) and 76 clinical isolates of diverse origin. Most results from the two approaches were in agreement, but some discrepancies were discerned. Advantages over previous phenotypic and genetic methods are discussed.