Synthetic algal-bacteria consortia for space-efficient microalgal growth in a simple hydrogel system
Synthetic algal-bacteria consortia for space-efficient microalgal growth in a simple hydrogel system
复制标题
DOI：
10.1007/s10811-021-02528-7
复制
发表时间：
2021-03
影响因子：
3.3
通讯作者：
N. Martin;T. Bernat;J. Dinasquet;Andrea Stofko;A. Damon;D. Deheyn;F. Azam;J. Smith;M. P. Davey;Alison G. Smith;S. Vignolini;D. Wangpraseurt
中科院分区：
文献类型：
--
作者：
N. Martin;T. Bernat;J. Dinasquet;Andrea Stofko;A. Damon;D. Deheyn;F. Azam;J. Smith;M. P. Davey;Alison G. Smith;S. Vignolini;D. Wangpraseurt
光合微藻是一种有吸引力的食物、燃料或营养品来源，但微藻的商业化生产受到低空间效率的限制。在本研究中，我们开发了一个简单的光合水凝胶系统，培养的绿色microphores，Marinichlorella kaistiaeKAS 603，连同一个新的菌株的细菌，Erythrophorasp。我们使用叶绿素荧光测定法、微传感和生物光学测量的组合来测试水凝胶中的共培养物的性能。我们的研究结果表明，在藻-细菌水凝胶的生长速率提高了约三倍，单独与藻类的水凝胶。基于叶绿素荧光光谱的光曲线发现，藻类细菌水凝胶的电子传输速率增强约20%相比，藻类水凝胶的中间辐照水平。我们还表明，生活水凝胶在不同的环境条件下是稳定的，当暴露于天然海水。我们的研究提供了一个潜在的生物启发的解决方案，限制了生物技术应用中微藻的空间有效培养的问题。
Photosynthetic microalgae are an attractive source of food, fuel, or nutraceuticals, but commercial production of microalgae is limited by low spatial efficiency. In the present study we developed a simple photosynthetic hydrogel system that cultivates the green microalga,Marinichlorella kaistiaeKAS603, together with a novel strain of the bacteria,Erythrobactersp. We tested the performance of the co-culture in the hydrogel using a combination of chlorophyll-afluorimetry, microsensing, and bio-optical measurements. Our results showed that growth rates in algal–bacterial hydrogels were about threefold enhanced compared to hydrogels with algae alone. Chlorophyll-afluorimetry–based light curves found that electron transport rates were enhanced about 20% for algal–bacterial hydrogels compared to algal hydrogels for intermediate irradiance levels. We also show that the living hydrogel is stable under different environmental conditions and when exposed to natural seawater. Our study provides a potential bio-inspired solution for problems that limit the space-efficient cultivation of microalgae for biotechnological applications.