Kinetics of uptake and metabolism by endothelial cell from indicator dilution data.
Kinetics of uptake and metabolism by endothelial cell from indicator dilution data.
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根据指示剂稀释数据，内皮细胞摄取和代谢的动力学。
DOI：
10.1007/bf02364055
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发表时间：
1987
影响因子：
3.8
通讯作者：
Dawson,CA
中科院分区：
文献类型：
--
作者：
Linehan,JH;Bronikowski,TA;Dawson,CA
某些底物在可饱和过程中被肺内皮细胞快速吸收和/或代谢。当这种底物和参考指示剂被包括在注射到流入肺的血液中的推注中时，在肺静脉流出中测量的提取率E（t）曲线相对于参考指示剂曲线是不对称的。如果团中包含足够量的底物，则提取曲线向上凹。E（t）曲线的形状包含关于化学-物理过程的信息，所述化学-物理过程控制底物在其单次通过肺期间的命运。为了解释的形状，计算机模拟被用来分别说明的影响的摄取到细胞中的基板，从细胞的返回流量的基板，饱和现象的提取过程中，和灌注的毛细管的异质性。最后，一个简单的分析方法估计器官动力学参数的提取过程。
Certain substrates are rapidly taken up and/or metabolized by pulmonary endothelial cells in a saturable process. When such a substrate and a reference indicator are included in a bolus which is injected into the blood flowing into the lung, the extraction ratio, E(t), curves measured in the pulmonary venous outflow are asymmetric with respect to the reference indicator curve. If a sufficient quantity of substrate is included in the bolus, the extraction curves are concave upward. The shapes of the E(t) curves contain information regarding the chemical-physical processes which govern the fate of the substrate during its single passage through the lung. To interpret the shapes, computer simulations are used to illustrate separately the effects of the uptake of substrate into the cell, the returning flux of the substrate from the cell, the saturation phenomena of the extraction process, and the perfusion heterogeneity of the capillaries. Lastly, a simple analytical method for estimating the organ kinetic parameters of the extraction process is presented.