The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction
The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction
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DOI：
10.1016/j.biomaterials.2008.10.006
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发表时间：
2009-02-01
期刊：
影响因子：
14
通讯作者：
Yoo, James J.
中科院分区：
文献类型：
--
作者：
Tillman, Bryan W.;Yazdani, Saami K.;Yoo, James J.
为了避免假体血管移植的并发症，工程血管构建体被研究作为血管重建的替代方法。用于血管组织工程的支架仍然是这些努力的基石，然而许多目前可用的选择受到不一致，血管细胞粘附性差或生物力学性能不足等问题的限制。在本研究中，我们研究了PCL/胶原支架是否能够在维持兔主动脉-髂旁路模型通畅的同时支持细胞生长和承受生理条件。我们的研究结果表明，电纺丝支架在生理条件下支持血管细胞的粘附和生长，内皮化的移植物在暴露于血液中时抵抗血小板的粘附。当植入体内时，这些支架能够在植入1个月后保持其结构完整性，这是通过连续超声检查证明的。此外，在回收时，这些支架继续保持与天然动脉相当的生物力学强度。本研究提示，结合血管细胞的电纺丝支架可能成为替代人工血管移植物的血管重建方法。(C) 2008 Elsevier Ltd版权所有。
To avoid complications of prosthetic vascular grafts, engineered vascular constructs have been investigated as an alternative for vascular reconstruction. The scaffolds for vascular tissue engineering remain a Cornerstone of these efforts and yet many Currently available options are limited by issues of inconsistency, poor adherence Of Vascular cells, or inadequate biomechanical properties. In this Study, we investigated whether PCL/collagen scaffolds could Support cell growth and withstand physiologic conditions while maintaining patency in a rabbit aortoiliac bypass model. Our results indicate that electrospun scaffolds support adherence and growth of vascular cells under physiologic conditions and that endothelialized grafts resisted adherence of platelets when exposed to blood. When implanted in vivo, these scaffolds were able to retain their structural integrity over 1 month of implantation as demonstrated by serial ultrasonography. Further, at retrieval, these scaffolds continued to maintain biomechanical Strength that was comparable to native artery. ThiS Study Suggests that electrospun scaffolds combined with vascular cells may become an alternative to prosthetic vascular grafts for vascular reconstruction. (C) 2008 Elsevier Ltd. All rights reserved.