Controlled immobilization of acetylcholinesterase on improved hydrophobic gold nanoparticle/Prussian blue modified surface for ultra-trace organophosphate pesticide detection.
Controlled immobilization of acetylcholinesterase on improved hydrophobic gold nanoparticle/Prussian blue modified surface for ultra-trace organophosphate pesticide detection.
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DOI：
10.1016/j.bios.2011.06.020
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发表时间：
2011-09
影响因子：
12.6
通讯作者：
Shuo Wu;Xiaoqin Lan;Wei Zhao;Yuping Li;Lihui Zhang;Hainan Wang;Mei Han;Shengyang Tao
中科院分区：
文献类型：
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作者：
Shuo Wu;Xiaoqin Lan;Wei Zhao;Yuping Li;Lihui Zhang;Hainan Wang;Mei Han;Shengyang Tao
将乙酰胆碱酯酶（AChE）控制固定在金纳米颗粒/聚二甲基二烯丙基氯化铵保护的普鲁士蓝（Au-PDDA-PB）纳米复合修饰电极表面，制备了一种用于有机磷农药检测的超灵敏安培型乙酰胆碱酯酶（AChE）生物传感器。Au-PDDA-PB膜不仅能增强电子传递，而且具有较大的表面积，是一种很好的酶固定化基质。此外，通过调整金纳米粒子与PDDA-PB的比例，可以很好地控制Au-PDDA-PB纳米复合材料在25.6 ~ 78.1°的静态水接触角范围内的表面亲水性。在优化后的疏水表面上，乙酰胆碱酯酶的取向具有良好的活性和稳定性，这已被电化学方法证实。利用Au-PDDA-PB纳米复合材料的优势以及乙酰胆碱酯酶良好的活性和稳定性，该生物传感器对典型高毒有机磷的灵敏度显著提高，线性范围宽（1.0 ~ 1000pg/mL和1.0 ~ 10ng/mL），超低检出限为0.8pg/mL。该传感器精度高，重复性好，稳定性好。因此，该策略可能为蛋白质的控制固定和高灵敏度生物传感器的设计提供有用的信息。
An ultrasensitive amperometric acetylcholinesterase (AChE) biosensor was fabricated by controlled immobilization of AChE on gold nanoparticles/poly(dimethyldiallylammonium chloride) protected Prussian blue (Au–PDDA–PB) nanocomposite modified electrode surface for the detection of organophorous pesticide. The Au–PDDA–PB membrane served as an excellent matrix for the immobilization of enzyme, which not only enhanced electron transfer but also possessed a relatively large surface area. In addition, the surface hydrophilicity of the Au–PDDA–PB nanocomposite was finely controlled in the static water contact angle range of 25.6–78.1° by adjusting the ratio of gold nanoparticles to PDDA–PB. On an optimized hydrophobic surface, the AChE adopts an orientation with both good activity and stability, which has been proven by electrochemical methods. Benefit from the advantages of the Au–PDDA–PB nanocomposite and the good activity and stability of AChE, the biosensor shows significantly improved sensitivity to monocrotophos, a typical highly toxic organophorous pesticide, with wide linear range (1.0–1000pg/mL and 1.0–10ng/mL) and an ultra-low detection limit of 0.8pg/mL. The biosensor exhibits accuracy, good reproducibility and stability. This strategy may therefore provide useful information for the controlled immobilization of protein and the design of highly sensitive biosensors.