USE OF DEUTERIUM LABELED GLUCOSE IN EVALUATING THE PATHWAY OF HEPATIC GLYCOGEN-SYNTHESIS
USE OF DEUTERIUM LABELED GLUCOSE IN EVALUATING THE PATHWAY OF HEPATIC GLYCOGEN-SYNTHESIS
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DOI：
10.1016/0006-291x(89)90024-7
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发表时间：
1989-03-15
影响因子：
3.1
通讯作者：
JONES, AD
中科院分区：
文献类型：
--
作者：
GOODMAN, MN;MASUOKA, LK;JONES, AD
本文用氢标记葡萄糖研究了大鼠空腹-再灌胃过程中肝糖原合成途径。用核磁共振和质谱仪测定肝糖原中的氚富集量。空腹大鼠灌胃给药60分钟后，门静脉血中充盈了氢化葡萄糖。尽管如此，结合到肝糖原中的葡萄糖分子中只有不到一半含有氘。从葡萄糖中丢失的氚标记与肝糖原的合成是一致的，这是一条需要葡萄糖优先代谢的间接途径。氢标记葡萄糖的使用可能成为研究肝糖原代谢的有用的探针。它的应用也可能通过一种非侵入性的手段在人类肝糖原代谢的研究中得到应用。
Deuterium labelled glucose has been used to study the pathway of hepatic glycogen synthesis during the fasted-refed transition in rats. Deuterium enrichment of liver glycogen was determined using nuclear magnetic resonance as well as mass spectroscopy. Sixty minutes after oral administration of deuterated glucose to fasted rats, the portal vein blood was fully enriched with deuterated glucose. Despite this, less than half of the glucose molecules incorporated into liver glycogen contained deuterium. The loss of deuterium label from glucose is consistent with hepatic glycogen synthesis by an indirect pathway requiring prior metabolism of glucose. The use of deuterium labelled glucose may prove to be a useful probe to study hepatic glycogen metabolism. Its use may also find application in the study of liver glycogen metabolism in humans by a noninvasive means.