Identification of CDP-Archaeol Synthase, a Missing Link of Ether Lipid Biosynthesis in Archaea
Identification of CDP-Archaeol Synthase, a Missing Link of Ether Lipid Biosynthesis in Archaea
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DOI：
10.1016/j.chembiol.2014.07.022
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发表时间：
2014-10-23
影响因子：
--
通讯作者：
Driessen, Arnold J. M.
中科院分区：
文献类型：
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作者：
Jain, Samta;Caforio, Antonella;Driessen, Arnold J. M.
微囊膜脂质组成与细菌和真核生物不同，由醚化到甘油碳的类异戊二烯链组成。这些脂质的生物合成知之甚少。在这里，我们鉴定并表征了古细菌膜蛋白CDP-archaeol合酶（汽车），该合酶催化核苷酸转移到其特定的古细菌脂质底物，导致形成CDP-activated前体（CDP-archaeol），其上附着有极性头基。汽车的发现使整个古生菌脂质生物合成途径在体外重建，从简单的类异戊二烯积木和使用一套五个纯化的酶。我们描述的古细菌脂质的无细胞合成策略为古细菌脂质生物化学的研究打开了机会。此外，这里报道的古细菌脂质生物合成的见解允许解决古细菌和细菌之间的脂质划分的进化假说。
Archaeal membrane lipid composition is distinct from Bacteria and Eukarya, consisting of isoprenoid chains etherified to the glycerol carbons. Biosynthesis of these lipids is poorly understood. Here we identify and characterize the archaeal membrane protein CDP-archaeol synthase (CarS) that catalyzes the transfer of the nucleotide to its specific archaeal lipid substrate, leading to the formation of a CDP-activated precursor (CDP-archaeol) to which polar head groups are attached. The discovery of CarS enabled reconstitution of the entire archaeal lipid biosynthesis pathway in vitro, starting from simple isoprenoid building blocks and using a set of five purified enzymes. The cell free synthetic strategy for archaeal lipids we describe opens opportunity for studies of archaeal lipid biochemistry. Additionally, insights into archaeal lipid biosynthesis reported here allow addressing the evolutionary hypothesis of the lipid divide between Archaea and Bacteria.