5-Arylvinyl-2,2'-bipyridyls: Bright "push-pull" dyes as components in fluorescent indicators for zinc ions.
5-Arylvinyl-2,2'-bipyridyls: Bright "push-pull" dyes as components in fluorescent indicators for zinc ions.
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DOI：
10.1016/j.jphotochem.2015.05.008
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发表时间：
2015-10-01
期刊：
影响因子：
--
通讯作者：
Clark RJ
中科院分区：
文献类型：
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作者：
Zhu L;Younes AH;Yuan Z;Clark RJ
本文综述了芳基乙烯基联吡啶 (AVB) 的锌 (II) 依赖性光物理性质，AVB 是一类 2,2'-联吡啶和芳基电子共轭的氟离子载体。 AVB 的锌 (II) 结合可能会导致荧光离子载体的发射红移，而不会降低其荧光量子产率。这一观察结果可以用电子供体-π桥-电子受体“推拉”荧光团的激发态模型来解释，其中双吡咯部分充当电子受体，锌(II)配位增强了其电子亲和力。含联吡啶的氟离子载体（例如 AVB）对锌 (II) 的光谱敏感性可用于制备该离子的荧光指示剂。在一些情况下，AVB 部分被掺入荧光异二位配体中，因此锌 (II) 浓度在相对较大范围内的变化可以与荧光强度或颜色的变化相关。 AVB 荧光离子载体还用于引入分子内福斯特共振能量转移 (FRET) 策略，用于创建具有高光稳定性和窄发射带的锌 (II) 指示剂，这是荧光显微镜中使用的染料的两个所需特性。
This article reviews the zinc(II)-dependent photophysical properties of arylvinylbipyridines (AVBs), a class of fluoroionophores in which 2,2′-bipyridyl and an aryl moiety are electronically conjugated. Zinc(II) binding of an AVB may lead to an emission bathochromic shift of the fluoroionophore without diminishing its fluorescence quantum yield. This observation can be explained using the excited state model of electron donor–π bridge–electron acceptor “push–pull” fluorophores, in which the bipy moiety acts as an electron acceptor, and zinc(II)-coordination strengthens its electron affinity. The spectral sensitivity of bipy-containing fluoroionophores, such as AVBs, to zinc(II) can be exploited to prepare fluorescent indicators for this ion. In several cases, AVB moieties are incorporated in fluorescent heteroditopic ligands, so that the variation of zinc(II) concentration over a relatively large range can be correlated to fluorescence changes in either intensity or color. AVB fluoroionophores are also used to introduce an intramolecular Förster resonance energy transfer (FRET) strategy for creating zinc(II) indicators with high photostability and a narrow emission band, two desired characteristics of dyes used in fluorescence microscopy.