The functions of an apple cytosolic malate dehydrogenase gene in growth and tolerance to cold and salt stresses.
The functions of an apple cytosolic malate dehydrogenase gene in growth and tolerance to cold and salt stresses.
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DOI：
10.1016/j.plaphy.2010.12.009
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发表时间：
2011-03
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影响因子：
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通讯作者：
Yuxin Yao;Q. Dong;H. Zhai;C. You;Y. Hao
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文献类型：
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作者：
Yuxin Yao;Q. Dong;H. Zhai;C. You;Y. Hao
众所周知，胞质中依赖NAD的苹果酸脱氢酶(CyMDH；L-苹果酸：NAD-氧化还原酶；EC 1.1.1.37)是胞质中合成苹果酸的关键酶。关于CyMDH在生长和压力耐受性方面的研究尚不清楚。在这里，我们描述了苹果cyMDH基因(MdcyMDH，GenBank ID：DQ221207)在生长和耐冷性和盐胁迫中的作用。MdcyMDH转录本在生长旺盛的苹果组织、器官和悬浮细胞中高度积累。此外，MdcyMDH对低温和盐胁迫敏感。在苹果愈伤组织和番茄中，MdcyMDH的过表达有利于细胞生长和植株的生长，并增强了对胁迫的耐受性。综上所述，我们的结果表明，MdcyMDH参与了植物和细胞的生长以及对低温和盐胁迫的耐受性。
It is well-known that cytosolic NAD-dependent malate dehydrogenase (cyMDH; l-malate:NAD-oxidoreductase; EC 1.1.1.37) is an enzyme crucial for malic acid synthesis in the cytosol. Nothing is known about cyMDH in growth and stress tolerance. Here we characterised the role of the apple cyMDH gene (MdcyMDH, GenBank ID: DQ221207) in growth and tolerance to cold and salt stresses. MdcyMDH transcripts were highly accumulated in vigorously growing apple tissues, organs and suspension cells. In addition, MdcyMDH was sensitive to cold and salt stresses. MdcyMDH overexpression favourably contributed to cell and plant growth and conferred stress tolerance both in the apple callus and tomato. Taken together, our results indicated that MdcyMDH is involved in plant and cell growth as well as the tolerance to cold and salt stresses.