Simultaneous monitoring of faecal indicators and harmful algae using an in‐situ autonomous sensor
Simultaneous monitoring of faecal indicators and harmful algae using an in‐situ autonomous sensor
复制标题
使用原位自主传感器同时监测粪便指标和有害藻类
DOI：
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发表时间：
2015
影响因子：
2.4
通讯作者：
C. Scholin
中科院分区：
文献类型：
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作者：
K. Yamahara;K. Yamahara;Elif Demir;C. Preston;R. Marin;D. Pargett;B. Roman;S. Jensen;J. Birch;Alexandria B. Boehm;C. Scholin
粪便指示细菌（FIB）和有害藻华（HABs）威胁着全球沿海社区的健康和经济。新兴的自动化采样技术与分子分析技术相结合，可以快速检测现场微生物，从而改善资源管理和公共卫生决策。我们使用机器人设备环境样本处理器（ESP）评估了这一概念。ESP可自动进行原位样本采集、核酸提取和分子分析。在这里，ESP测量并报告FIB（肠球菌属）的浓度，微生物来源追踪标记物（人类特异性拟杆菌目）和HAB种（拟菱形藻属）在圣克鲁斯市政码头（美国加利福尼亚州圣克鲁斯）进行为期45天的部署。FIB和HAB均从单个原位收集的水样中计数。原位qPCR效率范围为86%至105%，而部署期间的定量限为10个拷贝反应-1。肠球菌（类杆菌属中的人类特异性标志物）的浓度没有观察到差异，与传统手工取样法相比，P > 0. 05。分析结果可在样品采集后数小时内通过互联网获取，这证明了当天公布当前水质状况的可行性。
Faecal indicator bacteria (FIB) and harmful algal blooms (HABs) threaten the health and the economy of coastal communities worldwide. Emerging automated sampling technologies combined with molecular analytical techniques could enable rapid detection of micro‐organisms in‐situ, thereby improving resource management and public health decision‐making. We evaluated this concept using a robotic device, the Environmental Sample Processor (ESP). The ESP automates in‐situ sample collection, nucleic acid extraction and molecular analyses. Here, the ESP measured and reported concentrations of FIB (Enterococcus spp.), a microbial source‐tracking marker (human‐specific Bacteriodales) and a HAB species (Psuedo‐nitzschia spp.) over a 45‐day deployment on the Santa Cruz Municipal Wharf (Santa Cruz, CA, USA). Both FIB and HABs were enumerated from single in‐situ collected water samples. The in‐situ qPCR efficiencies ranged from 86% to 105%, while the limit of quantifications during the deployment was 10 copies reaction−1. No differences were observed in the concentrations of enterococci, the human‐specific marker in Bacteroidales spp., and P. australis between in‐situ collected sample and traditional hand sampling methods (P > 0·05). Analytical results were Internet‐accessible within hours of sample collection, demonstrating the feasibility of same‐day public notification of current water quality conditions.
影响因子：
56.9
作者：
DELONG, EF;WICKHAM, GS;PACE, NR
通讯作者：
PACE, NR