High-uptake areas on positron emission tomography with the hypoxic radiotracer (18)F-FRP170 in glioblastomas include regions retaining proliferative activity under hypoxia.
High-uptake areas on positron emission tomography with the hypoxic radiotracer (18)F-FRP170 in glioblastomas include regions retaining proliferative activity under hypoxia.
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DOI：
10.1007/s12149-015-0951-0
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发表时间：
2015-05
影响因子：
2.6
通讯作者：
Takai Y
中科院分区：
文献类型：
--
作者：
Beppu T;Sasaki T;Terasaki K;Saura H;Mtsuura H;Ogasawara K;Sasaki M;Ehara S;Iwata R;Takai Y
目的是用低氧细胞放射性示踪剂1-(2-[18F]fluoro-1-[hydroxymethyl]ethoxy)methyl-2-nitroimidazole(FRP170)在正电子发射断层扫描上评价高摄取区的增殖活性。13例胶质母细胞瘤患者在肿瘤切除前行FRP170PET检查。在手术中，肿瘤标本是从FRP170的高摄取区(高摄取区)和相对低摄取区(低摄取区)对应的立体定向获得的。比较Ki-67和低氧诱导因子-1α在HUA和LUA中的表达。HUA组HIF-1α指数显著高于LuA组。相比之下，平均Ki-67指数在HUA和LuA之间没有显著差异。HIF-1α指数的研究结果清楚地表明，FRP170PET上的HUA代表胶质母细胞瘤的缺氧区。然而，Ki-67指数的发现表明，FRP170PET上的HUA包括与组织缺氧无关的保持增殖活性的区域。
The aim was to evaluate the proliferative activity of high-uptake areas on positron emission tomography (PET) with the hypoxic cell radiotracer, 1-(2-[18F]fluoro-1-[hydroxymethyl]ethoxy)methyl-2-nitroimidazole (FRP170). Thirteen patients with glioblastoma underwent FRP170 PET before tumor resection. During surgery, tumor specimens were stereotaxically obtained from regions corresponding to high (high-uptake areas, HUAs) and relatively low (low-uptake areas, LUAs) accumulation of FRP170. We compared immunohistochemical staining for Ki-67 and hypoxia-inducible factor (HIF)-1α between HUA and LUA. HIF-1α index was significantly higher in HUAs than in LUAs. In contrast, mean Ki-67 indices did not differ significantly between HUAs and LUAs. Findings for HIF-1α index clearly indicated that HUAs on FRP170 PET represented hypoxic regions in glioblastoma. However, findings of Ki-67 index suggest that HUAs on FRP170 PET include regions retaining proliferative activity regardless of tissue hypoxia.