HESRG: a novel biomarker for intracranial germinoma and embryonal carcinoma
HESRG: a novel biomarker for intracranial germinoma and embryonal carcinoma
复制标题
HESRG：颅内生殖细胞瘤和胚胎癌的新型生物标志物
DOI：
10.1007/s11060-011-0673-7
复制
发表时间：
2012-01-01
影响因子：
3.9
通讯作者：
Ren, Caiping
中科院分区：
文献类型：
--
作者：
Wanggou, Siyi;Jiang, Xingjun;Ren, Caiping
新的干细胞相关基因HESRG是我们小组首次发现的，其在人类肿瘤中的表达模式仍然未知。本研究采用RT-PCR方法系统研究了HESRG在不同类型颅内肿瘤中的表达，发现HESRG仅在生殖细胞瘤和胚胎性癌中表达，而在其他类型脑肿瘤中几乎不表达。实时荧光定量PCR结果进一步证实了这种表达模式。随后，我们用免疫组织化学方法检测了134例颅内非生殖细胞肿瘤和64例颅内生殖细胞肿瘤。我们的研究结果表明，HESRG强烈和弥漫性表达在颅内生殖细胞瘤和胚胎癌以及人胚胎干细胞的肿瘤细胞核。在任何其他类型的颅内肿瘤中均未观察到阳性染色信号。在生殖细胞瘤中，31例中有25例表现出强（3+）表达，4例表现出中度（2+）免疫染色，其余2例表现出弱（1+）免疫染色。在胚胎癌中，9例中6例显示强烈（3+）免疫染色，9例中3例显示中度（2+）免疫染色。这些结果表明，HESRG是一种新的，敏感和特异性的颅内生殖细胞瘤和胚胎性癌的生物标志物。
The novel stem cell-related gene, HESRG, was first identified by our group, and its expression pattern in human tumors remains unknown. In this study, we used RT-PCR to systematically investigate the expression of HESRG in various types of intracranial tumors and found that HESRG was expressed only in germinoma and embryonal carcinoma, but hardly at all in other types of brain tumors. Real-time PCR results further confirmed this expression pattern. Subsequently, we tested 134 intracranial non-germ cell tumors and 64 intracranial germ cell tumors by immunohistochemistry. Our results showed that HESRG was expressed strongly and diffusively in the nuclei of tumor cells in intracranial germinoma and embryonal carcinoma as well as in human embryonic stem cells. No positive staining signal was observed in any other type of intracranial tumors. In germinomas, 25 of 31 showed intensive (3+) expression, four cases showed moderate (2+) immunostaining and the remaining 2 cases showed weak (1+) immunostaining. In embryonal carcinoma, 6 of 9 showed intensive (3+) immunostaining and 3 of 9 showed moderate (2+) immunostaining. These results suggest that HESRG is a novel, sensitive and specific biomarker for intracranial germinoma and embryonal carcinoma.