Characterization of starch-accumulating duckweeds, Wolffia globosa, as renewable carbon source for bioethanol production
Characterization of starch-accumulating duckweeds, Wolffia globosa, as renewable carbon source for bioethanol production
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DOI：
10.1016/j.bcab.2016.03.006
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发表时间：
2016-04-01
影响因子：
4
通讯作者：
Shimizu, Sakayu
中科院分区：
文献类型：
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作者：
Fujita, Tomohiro;Nakao, Eiji;Shimizu, Sakayu
用HYPONeX研究了两种浮萍(球状浮萍J和浮萍B)在不同营养条件下的生长和淀粉积累能力。浮萍J和B在富营养条件下(5000倍稀释的HYPONeX溶液)比在低营养条件(80000倍稀释的HYPONeX溶液)下生长得更好。在淀粉积累方面，营养丰富的浮萍J积累的淀粉较多，营养较差的浮萍B积累的淀粉较多。在营养丰富的条件下，培养1周后，浮萍J的干重增加了约5.1倍，积累的淀粉含量约为干浮萍的22%(w/w)。在营养条件较差的情况下，培养1周后，浮萍B的干重增加了约5.0倍，积累的淀粉含量约为干浮萍的28%(w/w)。在此基础上，利用酿酒酵母NBRC0224对浮萍生产乙醇进行了研究。以浮萍J为例，以30%(w/v)的浮萍为原料，用20 mM尿素或0.1%酵母膏和30 mM硫酸铵处理，制得69g/L乙醇。以浮萍B为例，30%(w/v)的浮萍在不加氮源的情况下，可生产30g/L乙醇。总之，球藻浮萍被发现是一种很有前途的可再生碳源，可用于生产第三代生物乙醇。(C)2016爱思唯尔有限公司。保留所有权利。
The growth and starch accumulation ability of two types of duckweeds (Wolffia globosa), designated as duckweeds J and B, respectively, were investigated under different nutrition conditions using HYPONeX. Both duckweeds J and B grew better in rich nutrient condition (5,000-fold diluted HYPONeX solution) than in poor nutrient condition (80,000-fold diluted HYPONeX solution). In terms of starch accumulation, duckweed J accumulated more starch in the rich nutrient condition, whereas duckweed B accumulated more starch in the poor nutrient condition. In the rich nutrient condition, the dry weight of duckweed J increased by about 5.1 folds and the accumulated starch content was about 22% (w/w) of dry duckweed after 1-week cultivation. In the poor nutrient condition, the dry weight of duckweed B increased by about 5.0 folds and the accumulated starch content was about 28% (w/w) of dry duckweed after 1-week cultivation. Furthermore, ethanol production from the duckweeds was investigated using Saccharomyces cerevisiae NBRC0224. The most effective pretreatment of duckweeds for ethanol production was treatment with 1% hydrogen peroxide for 1 h, followed by treatment with 1% sodium hydroxide for 1 h. In the case of the duckweed J, 69 g/L ethanol was produced from 30% (w/v) of the pretreated duckweed with 20 mM urea or 0.1% yeast extract and 30 mM ammonium sulfate. In the case of the duckweed B, 30 g/L ethanol was produced from 30% (w/v) of the non-pretreated duckweed without nitrogen source. In conclusion, the duckweeds, W. globosa, were found to be a promising renewable carbon source for the production of third-generation bioethanol. (C) 2016 Elsevier Ltd. All rights reserved.