No involvement of APC gene mutations in ulcerative colitis–associated rat colon carcinogenesis induced by 1‐hydroxyanthraquinone and methylazoxymethanol acetate
No involvement of APC gene mutations in ulcerative colitis–associated rat colon carcinogenesis induced by 1‐hydroxyanthraquinone and methylazoxymethanol acetate
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APC基因突变与1-羟基蒽醌和甲基偶氮甲醇乙酸酯诱导的溃疡性结肠炎相关大鼠结肠癌的发生无关
作者：
M. Suzui;T. Ushijima;N. Yoshimi;H. Nakagama;A. Hara;T. Sugimura;M. Nagao;H. Mori
通过使用1‐羟基蒽醌（1‐HA）引起结肠黏膜严重炎症，建立了人类溃疡性结肠炎（UC）大鼠模型。1‐HA还对大鼠结肠中甲基氧化甲醇（MAM）醋酸酯的致癌性具有协同作用。本研究采用聚合酶链反应(PCR) -单链构象多态性（SSCP）和PCR -限制性内切- SSCP分析，检测了4例雄性F344大鼠腺瘤和16例雄性F344大鼠APC基因编码区和编码外显子侧内含子的突变。没有发现突变。这些结果，加上我们之前在相同肿瘤中相对缺乏Ki - ras基因突变的观察，与在人类UC相关结肠癌中发现的相似，表明这两个系统中存在共同的途径，尽管它们在p53突变的含义上不同。因此，该模型可能对与APC突变或Ki - ras或p53突变无关的人类炎症性肠病结肠癌的研究具有一定的相关性和应用价值。中华医学杂志，20(2):389 - 393,1997。©1997 Wiley‐Liss, Inc。
A rat model for human ulcerative colitis (UC) has been developed by using 1‐hydroxyanthraquinone (1‐HA) to cause severe inflammation of colonic mucosa. 1‐HA also has synergistic effects on the carcinogenicity of methylazoxymethanol (MAM) acetate in the rat colon. In this study, four adenomas and 16 adenocarcinomas induced in male F344 rats by 1‐HA and MAM acetate were examined for mutations in the entire coding regions and introns flanking coding exons of the APC gene by polymerase chain reaction (PCR)–single strand conformation polymorphism (SSCP) and PCR‐restriction‐SSCP analyses. No mutations were found. These results, together with our previous observations of a relative lack of Ki‐ras gene mutations in the same tumors, are similar to those found in human UC‐associated colon cancer, suggest a common pathway in these two systems, although they are different in their implication of p53 mutations. Therefore, this model may have some relevance and application to the study of colon cancer in human inflammatory bowel disease, which is not associated with APC mutations or with Ki‐ras or p53 mutations. Mol. Carcinog. 20:389–393, 1997. © 1997 Wiley‐Liss, Inc.
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影响因子：
29.4
作者：
Mark Redston;N. Papadopoulos;Carlos Caldas;K. Kinzler;Scott E. Kern
通讯作者：
Mark Redston;N. Papadopoulos;Carlos Caldas;K. Kinzler;Scott E. Kern
影响因子：
11.2
作者：
L. Tarmin;Jing Yin;N. Harpaz;M. Kozam;Jeroen Noordzij;Lilian B. Antonio;H. Jiang;O. Chan;K. Cymes;S. Meltzer
通讯作者：
L. Tarmin;Jing Yin;N. Harpaz;M. Kozam;Jeroen Noordzij;Lilian B. Antonio;H. Jiang;O. Chan;K. Cymes;S. Meltzer
通过聚合酶链式反应产物的直接测序测定人胃肠道发育不良中 c-Ki-ras 的激活。
影响因子：
11.2
作者：
Meltzer,SJ;Mane,SM;Wood,PK;Resau,JH;Newkirk,C;Terzakis,JA;Korelitz,BI;Weinstein,WM;Needleman,SW
通讯作者：
Needleman,SW
影响因子：
29.4
作者：
KERN, SE;REDSTON, M;KINZLER, KW
通讯作者：
KINZLER, KW
溃疡性结肠炎相关结直肠发育不良和癌症中的微卫星不稳定性。
影响因子：
11.2
作者：
Suzuki,H;Harpaz,N;Tarmin,L;Yin,J;Jiang,HY;Bell,JD;Hontanosas,M;Groisman,GM;Abraham,JM;Meltzer,SJ
通讯作者：
Meltzer,SJ