METHYLATION STATE OF POLY A-CONTAINING MESSENGER RNA FROM CULTURED HAMSTER CELLS
METHYLATION STATE OF POLY A-CONTAINING MESSENGER RNA FROM CULTURED HAMSTER CELLS
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DOI：
10.1093/nar/2.10.1653
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发表时间：
1975-01-01
影响因子：
14.9
通讯作者：
TAYLOR, RH
中科院分区：
文献类型：
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作者：
DUBIN, DT;TAYLOR, RH
培养的仓鼠（BHK-21）细胞中含有多聚腺苷酸的mRNA的甲基化状态进行了检查。在[甲基-3H]-蛋氨酸和32 Pi存在下孵育细胞20- 22小时，获得稳态标记的mRNA。该RNA的甲基化程度为每1000个核苷酸1.8个甲基，或平均每个分子4-5个甲基。甲基化残留物的性质通过酸性和碱性水解产物的纸层析和电泳、碱性水解产物和T2 RNase酶的DEAE纤维素层析以及通过检查样品进行“β-消除”的效果来确定。约一半的甲基出现在标准（“内部”）键中，10%作为m5 Cp残基，40%作为m6 Ap残基。其余的至少大部分发生在假定结构为m7 G（5′）ppp（Nm）p..的“封闭”5′-末端，其中Nm是Gm、m6 Am、Um或Cm。
The poly A-containing mRNA of cultured hamster (BHK-21) cells has been examined with regard to methylation status. Steady state-labeled mRNA was obtained by incubating cells for 20–22h in the presence of [methyl-3H]-methionine and32Pi. The degree of methylation of this RNA was 1.8 methyl groups per 1000 nucleotides, or 4–5 methyl groups on the average per molecule. The nature of the methylated residues was determined by paper chromatography and electrophoresis of acid and alkaline hydrolysates, by DEAE cellulose chromatography of alkaline hydrolysates and of T2 RNase digests, and by examining the effect of subjecting samples to “β-elimination.” Approx. half of the methyl groups occurred in standard (“internal”) linkage, 10% as m5Cp and 40% as m6Ap residues. The remainder occurred at least for the most part in “blocked” 5′-termini with the presumptive structure m7G(5′)ppp(Nm)p.., where Nm was Gm, m6Am, Um, or Cm.