Competitive annealing of multiple DNA origami: formation of chimeric origami
Competitive annealing of multiple DNA origami: formation of chimeric origami
复制标题
DOI：
10.1088/1367-2630/18/11/115001
复制
发表时间：
2016-11-17
影响因子：
3.3
通讯作者：
LaBean, Thomas H.
中科院分区：
文献类型：
--
作者：
Majikes, Jacob M.;Nash, Jessica A.;LaBean, Thomas H.
支架DNA折纸是一个强大的工具，以建立离散的纳米级物体在高产量。该策略确保在设计过程中，所需的纳米结构是所设计的DNA序列集的最小自由能态。尽管目标是最小自由能结构，但导致这种构象的折叠过程很难表征，尽管它已成为许多研究的主题。为了阐明分子折叠途径，本研究有意通过同时退火多个目标或母折纸结构的短纤维池，迫使竞争来挫败这些系统的折叠过程。这些竞争性的，同时退火的一个令人惊讶的结果是形成嵌合DNA折纸，继承结构区域，从两个亲本折纸。通过比较从母体折纸继承的区域，比较子结构的相对稳定性。这允许用典型的表征技术和材料检查折叠过程。然后使用退火曲线作为一种手段，快速生成预期行为的相图，作为短纤维过剩和母短纤维比的函数。这项初步研究表明，竞争性退火提供了一种令人兴奋的方法来创造各种新的纳米结构，并可用于检查各种结构基序的相对稳定性。
Scaffolded DNA origami are a robust tool for building discrete nanoscale objects at high yield. This strategy ensures, in the design process, that the desired nanostructure is the minimum free energy state for the designed set of DNA sequences. Despite aiming for the minimum free energy structure, the folding process which leads to that conformation is difficult to characterize, although it has been the subject of much research. In order to shed light on the molecular folding pathways, this study intentionally frustrates the folding process of these systems by simultaneously annealing the staple pools for multiple target or parent origami structures, forcing competition. A surprising result of these competitive, simultaneous anneals is the formation of chimeric DNA origami which inherit structural regions from both parent origami. By comparing the regions inherited from the parent origami, relative stability of substructures were compared. This allowed examination of the folding process with typical characterization techniques and materials. Anneal curves were then used as a means to rapidly generate a phase diagram of anticipated behavior as a function of staple excess and parent staple ratio. This initial study shows that competitive anneals provide an exciting way to create diverse new nanostructures and may be used to examine the relative stability of various structural motifs.