ELECTRON-MICROSCOPY OF HEPATITIS-B CORE ANTIGEN SYNTHESIZED IN ESCHERICHIA-COLI
ELECTRON-MICROSCOPY OF HEPATITIS-B CORE ANTIGEN SYNTHESIZED IN ESCHERICHIA-COLI
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DOI：
10.1038/296677a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
RICHMOND, JE
中科院分区：
文献类型：
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作者：
COHEN, BJ;RICHMOND, JE
乙型肝炎病毒 (HBV) 的 DNA 片段已插入质粒载体并克隆到大肠杆菌中1，据报道其中一些细菌克隆可合成乙型肝炎核心抗原 (HBcAg)1,2。我们的实验室和其他地方正在评估来自一种 HBcAg 合成克隆的衍生物的材料3,4作为血清学试剂用于各种测定（包括免疫电子显微镜）以检测抗 HBcAg 抗体。凝胶排阻色谱显示细菌 HBcAg 呈聚集形式3。我们现在报告E。大肠杆菌HBcAg 制剂含有小圆形颗粒，其外观与之前在从感染 HBV 的人类 5-7 和黑猩猩 8 中获得的材料中看到的病毒核心相似。
DNA fragments from hepatitis B virus (HBV) have been inserted into plasmid vectors and cloned inEscherichia coli1, and some of these bacterial clones have been reported to synthesize hepatitis B core antigen (HBcAg)1,2. Material from a derivative of one HBcAg-synthesizing clone is being evaluated in our laboratory and elsewhere3,4as a serological reagent for use in various assays, including immune electron microscopy, to detect anti-HBcAg antibody. It has been shown by gel exclusion chromatography that the bacterial HBcAg is in an aggregated form3. We now report that theE. coliHBcAg preparation contains Small round particles similar in appearance to the viral cores previously seen in material obtained from HBV-infected humans5–7and chimpanzees8.