Prenatal alcohol exposure and the effects of environmental enrichment on hippocampal dendritic spine density
Prenatal alcohol exposure and the effects of environmental enrichment on hippocampal dendritic spine density
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DOI：
10.1016/0741-8329(95)02049-7
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发表时间：
1996-03-01
期刊：
影响因子：
2.3
通讯作者：
Zajac, CS
中科院分区：
文献类型：
--
作者：
Berman, RF;Hannigan, JH;Zajac, CS
在产前暴露于酒精的大鼠中，研究了丰富环境对海马CAI区突触棘密度的影响。从妊娠第8天到第19天，通过胃内插管给予妊娠大鼠乙醇（6 g/kg/天），或给予等热量蔗糖。还使用未处理的对照组。断奶后，三组的后代在隔离（单独关在笼子里，不处理）或富集（用“玩具”饲养的组，处理）条件下饲养10周。然后处死动物，大脑高尔基体浸渍，CAI锥体细胞顶端和基底棘密度定量。在隔离动物中，对照组和酒精暴露组之间没有显着差异。在一般情况下，环境富集增加顶端或基底棘密度在未经处理和蔗糖对照。然而，在产前酒精暴露的动物，环境丰富并没有增加脊柱密度。由于环境富集作用于出生后，这些研究结果表明，产前酒精暴露的影响包括持续到成年早期的神经可塑性降低。海马神经解剖可塑性的这种降低可能与产前酒精暴露后发现的认知障碍有关。
The effects of environmental enrichment on synaptic spine density in hippocampal area CAI were examined in rats exposed prenatally to alcohol. Pregnant darns were given ethanol via intragastric intubation (6 g/kg/day) from gestational days 8 through 19, or given isocaloric sucrose. An untreated control group was also used. After weaning, offspring from the three groups were then reared for 10 weeks in either isolated (caged alone, not handled) or enriched (group housed with ''toys,'' handled) conditions. Animals were then sacrificed, the brains Golgi impregnated, and CAI pyramidal cell apical and basilar spine densities quantified. Among isolated animals there were no significant differences between control and alcohol-exposed groups. In general, environmental enrichment increased apical or basilar spine densities in untreated and sucrose controls. However, in prenatal alcohol-exposed animals, environmental enrichment did not increase spine densities. Because the environmental enrichment acted postnatally, these findings suggest that the effects of prenatal alcohol exposure include decreased neural plasticity enduring into early adulthood. Such a reduction in neuroanatomical plasticity in hippocampus may be associated with cognitive impairments found following prenatal alcohol exposure.