Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines.
Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines.
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恶性鼠淋巴肉瘤细胞系的凝集素附着变体的选择和体内特性。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
G. Nicolson
中科院分区：
文献类型：
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作者：
C. Reading;P. Belloni;G. Nicolson
恶性RAW 117淋巴肉瘤细胞系的变异体通过连续不粘附聚苯乙烯固定凝集素来选择。亲本小鼠淋巴肉瘤系RAW 117-P在悬浮液中生长，并被固定化伴刀豆球蛋白A（Con A）、蓖麻凝集素I（RCAI）、花生凝集素（PNA）或麦胚凝集素（WGA）迅速结合。在大多数细胞附着到凝集素取代的培养皿后，通过抽吸去除未附着的细胞（约1%）并传代培养。通过不粘附于固定凝集素的选择重复7至10次，并在BALB/c小鼠中测试所得细胞系以确定其恶性潜能。亲本系RAW 117-P在14天后每5 × 10 - 17 IV注射的细胞形成很少的（中值，0;范围，0-20）肝肿瘤集落，而在固定化Con A上选择10次的变体系（RAW 117 P Con d ′ o，）形成中值为95（范围，9->200）肝集落。RAW 117-P细胞选择固定的WGA，PNA，或RCAI上的非粘附显示肝肿瘤形成的增加相比，父母线的细胞。相比之下，RAW 117-H10，一种在体内选择10次用于肝定殖的变体系，形成了超过200个肝肿瘤集落，并且在固定化WGA上选择8次产生了仅形成1个（范围，0-4）肿瘤集落的中值的系（RAW 117-H10 WGA-8）。在固定化RCAI、Con A或PNA上选择的RAW 117-H10系的变体仍然形成超过200个肝肿瘤集落。1251-标记的凝集素结合研究表明在体内和一些固定的凝集素选择的线上的凝集素结合位点减少。用十二烷基硫酸钠聚丙烯酰胺凝胶自显影显示，在体内和固定化的Con A选择系中，Con A染色的主要条带（mol/wt = 70，000）减少。这些研究表明，细胞表面凝集素受体的改变可以改变体内的恶性行为。JNCI 64：
Variants of malignant RAW117 lymphosarcoma cell lines were selected by sequential nonadherence to polystyreneImmobilized lectlns. Parental murine lymphosarcoma line RAW117-P grew In suspension and was rapidly bound by Immobilized concanavalin A (Con A), Ricinus communis agglutinin I (RCAI), peanut agglutinin (PNA), or wheat germ agglutinin (WGA). After attachment of most cells to lectin-substituted petri dishes, the unattached cells «1%) were removed by aspiration and subcultured. Selection by nonadherence to Immobilized lectlns was repeated seven to ten times, and the resulting cell lines were tested in BALB/c mice to determine their malignant potentials. Parental line RAW117-P formed few (median, 0; range, 0-20) liver tumor colonies per 5x10' Iv Injected cells after 14 days, whereas a variant line selected ten times on Immobilized Con A (RAW117P Con d'o,) formed a median of 95 (range, 9->200) liver colonies. RAW117-P cells selected by nonadherence on immobilized WGA, PNA, or RCAI showed no increase in liver tumor formation compared to the cells of the parental line. In contrast, RAW117-H10, a variant line selected ten times In vivo for liver colonization, formed more than 200 liver tumor colonies, and selection eight times on Immobilized WGA yielded a line (RAW117-H10 WGA"8) that formed a median of only 1 (range, 0-4) tumor colony. Variants of the RAW117-H10 line selected on Immobilized RCAI, Con A, or PNA still formed more than 200 liver tumor colonies. 1251-labeled lectin-binding studies Indicated decreased lectin-binding sites on the In vivo and some of the immobilized lectin-selected lines. Autoradlographs of '''I-labeled Con A-stained sodium dodecyl sulfate polyacrylamide gels showed a decrease In the major Con A-staining band (mol wt, =70,000) In the in vivo and Immobilized Con A-selected lines. These studies Indicated that alterations In cell surface lectin receptors could modify in vivo malignant behavior.-JNCI 64: