H19 Functions as a ceRNA in Promoting Metastasis Through Decreasing miR-200s Activity in Osteosarcoma (Retracted article. See vol. 41, pg. 330, 2022)
H19 Functions as a ceRNA in Promoting Metastasis Through Decreasing miR-200s Activity in Osteosarcoma (Retracted article. See vol. 41, pg. 330, 2022)
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DOI：
10.1089/dna.2015.3171
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发表时间：
2016-05-01
影响因子：
3.1
通讯作者：
Cheng, Cai
中科院分区：
文献类型：
--
作者：
Li, Min;Chen, Hanwen;Cheng, Cai
骨肉瘤是世界上最常见的原发性骨肉瘤。骨肉瘤的预后差和复发率高，很大程度上是由于肺转移率高。据报道，H19在骨肉瘤进展中发挥潜在作用。然而，涉及H19的转移的确切分子机制仍不清楚。在本研究中，我们进行了功能获得和丧失的测定，发现H19促进骨肉瘤细胞的迁移和侵袭。此外，我们发现H19通过竞争性结合miR-200家族上调ZEB 1和ZEB 2促进转移。因此，我们的研究结果表明，H19在骨肉瘤转移的重要作用，并表明其在骨肉瘤治疗的潜在应用。
Osteosarcoma is the most common primary bone sarcoma around the world. The poor prognosis and high recurrence rate of osteosarcoma are largely due to the high rate of pulmonary metastasis. H19 has been reported to play a potential role in osteosarcoma progression. However, the exact molecular mechanism of metastasis involving H19 remains unclear. In the present study, we performed gain- and loss-of-function assays and found that H19 promotes migration and invasion in osteosarcoma cells. Furthermore, we showed that H19 promotes metastasis through upregulation of ZEB1 and ZEB2 by competitively binding the miR-200 family. Thus, our findings suggest important roles of H19 in osteosarcoma metastasis and indicate its potential application in osteosarcoma therapy.