Effect of DNA structure on the formation of collagen-DNA complex
Effect of DNA structure on the formation of collagen-DNA complex
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DOI：
10.1016/j.ijbiomac.2004.11.005
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发表时间：
2005-03-01
影响因子：
8.2
通讯作者：
Nishi, N
中科院分区：
文献类型：
--
作者：
Kaya, M;Toyama, Y;Nishi, N
利用质粒DNA制备的各种类型的DNA，线性和环状的完整双链DNA （ds.DNA）和双链DNA与单链DNA （ss.DNA）共存，通过浊度、透射电镜和激光共聚焦扫描显微镜研究了胶原-DNA复合物的结构和纤维形成。胶原-DNA复合体的纤维形成率明显取决于DNA结构。复合物中形成的原纤维结构在两组间有显著差异。DNA和DNA复合物与胶原蛋白。DNA和胶原蛋白在复合物中的空间分布表明，胶原-DNA相互作用的特征取决于DNA的形式。(c) 2004 Elsevier B.V.版权所有
Using various types of DNAs prepared from plasmid DNA, complete double-stranded DNA (ds.DNA) with linear and cyclic forms and double-stranded DNA coexisting with single-stranded DNA (ss.DNA), the structure and fibrillogenesis of the collagen-DNA complex were investigated by means of turbidity, transmission I electron microscopy, and confocal laser-scanning microscopy. The rate of fibrillogenesis of the collagen-DNA complex significantly depends on the DNA structure. The structure of the fibrils formed in the complexes showed a marked difference between the ds.DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms. (c) 2004 Elsevier B.V. All rights reserved.