SUMOylation of paraflagellar rod protein, PFR1, and its stage-specific localization in Trypanosoma cruzi.
SUMOylation of paraflagellar rod protein, PFR1, and its stage-specific localization in Trypanosoma cruzi.
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DOI：
10.1371/journal.pone.0037183
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Nara T
中科院分区：
文献类型：
--
作者：
Annoura T;Makiuchi T;Sariego I;Aoki T;Nara T
鞭毛虫原生动物寄生虫，克氏锥虫，是恰加斯病的病原体，由reduviid错误传播给人类。该虫在宿主和载体中均以多种形态存在，在T. Cruzi与阶段特异性蛋白质合成和降解密切相关。然而，负责这种协调细胞分化的具体分子机制尚不清楚。SUMO偶联系统在特异性蛋白表达中起着重要作用。于T. cruzi，SUMO化蛋白候选物的子集，以及SUMO的核定位。在这里，我们研究了相扑在T。克鲁兹T.内SUMO共有基序的定点突变分析。cruzi的SUMO分析表明，T. cruzi相扑可以。采用T. cruzi SUMO特异性抗体显示SUMO在上鞭毛体、后循环期和血流期的鞭毛上有核外定位。在短鞭毛细胞内无鞭毛体中，SUMO的核外分布与鞭毛基底相关，并随着无鞭毛体转化为锥鞭毛体而沿着鞭毛分布。我们研究了SUMO的鞭毛靶蛋白，并表明鞭毛旁杆蛋白PFR1是SUMO化的。这些结果表明，SUMO化与鞭毛的稳态在整个寄生虫的生活史，这可能在T。克鲁兹
The flagellate protozoan parasite, Trypanosoma cruzi, is a causative agent of Chagas disease that is transmitted by reduviid bugs to humans. The parasite exists in multiple morphological forms in both vector and host, and cell differentiation in T. cruzi is tightly associated with stage-specific protein synthesis and degradation. However, the specific molecular mechanisms responsible for this coordinated cell differentiation are unclear. The SUMO conjugation system plays an important role in specific protein expression. In T. cruzi, a subset of SUMOlylated protein candidates and the nuclear localization of SUMO have been shown. Here, we examined the biological roles of SUMO in T. cruzi. Site-directed mutagenesis analysis of SUMO consensus motifs within T. cruzi SUMO using a bacterial SUMOylation system revealed that T. cruzi SUMO can polymerize. Indirect fluorescence analysis using T. cruzi SUMO-specific antibody showed the extra-nuclear localization of SUMO on the flagellum of epimastigote and metacyclic and bloodstream trypomastigote stages. In the short-flagellate intracellular amastigote, an extra-nuclear distribution of SUMO is associated with basement of the flagellum and becomes distributed along the flagellum as amastigote transforms into trypomastigote. We examined the flagellar target protein of SUMO and show that a paraflagellar rod protein, PFR1, is SUMOylated. These findings indicate that SUMOylation is associated with flagellar homeostasis throughout the parasite life cycle, which may play an important role in differentiation of T. cruzi.
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影响因子：
3.9
作者：
ADROHER, FJ;OSUNA, A;LUPIANEZ, JA
通讯作者：
LUPIANEZ, JA
DOI：
10.1016/s0304-4165(00)00059-3
发表时间：
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作者：
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