Streptococcus suis in invasive human infections in Poland: clonality and determinants of virulence and antimicrobial resistance.
Streptococcus suis in invasive human infections in Poland: clonality and determinants of virulence and antimicrobial resistance.
复制标题
DOI：
10.1007/s10096-016-2616-x
复制
发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Sadowy E
中科院分区：
文献类型：
--
作者：
Bojarska A;Molska E;Janas K;Skoczyńska A;Stefaniuk E;Hryniewicz W;Sadowy E
本研究的目的是对由国家细菌性脑膜炎参考中心在波兰收集的猪链球菌人类侵袭性分离株进行分析。通过表型测试、多位点序列分型（MLST）、多位点可变数目串联重复序列（VNTR）分析（MLVA）方案的TR 9位点分析和SmaI消化DNA的脉冲场凝胶电泳（PFGE）对2000-2013年期间从21例患者中获得的分离株进行了研究。采用聚合酶链反应（PCR）检测毒力和耐药性的决定因素，并通过测序进行分析。所有分离株均代表序列1型（ST 1），建议为血清2型。PFGE和TR 9位点的分析分别允许区分4和17种类型。大多数分离株溶血和DNA酶阳性，其中约一半形成生物膜。猪溶血素、胞外蛋白因子、纤连蛋白结合蛋白、溶菌酶释放蛋白、表面抗原1、烯醇化酶、血清不透明因子和皮利基因在研究组中普遍存在，而没有分离株携带89 K致病岛的特征序列。所有分离株对青霉素、头孢噻肟、亚胺培南、氟沙星、氯霉素、利福平、庆大霉素、利奈唑胺、万古霉素和达托霉素敏感。5株分离株（24%）同时对红霉素、克林霉素和四环素不敏感，并携带泰特（O）和lnu（B）基因;对于1株分离株，还检测到lsa（E）和lnu（B）。从波兰人类侵入性感染中分离的猪链球菌属于该病原体的全球分布的克隆复合体，其毒力标记物丰富。这是首次报道在大豆中发现lsa（E）和lnu（B）抗性基因。猪。本文的在线版本（doi：10.1007/s10096-016-2616-x）包含补充材料，可供授权用户使用。
The purpose of this study was to perform an analysis of Streptococcus suis human invasive isolates, collected in Poland by the National Reference Centre for Bacterial Meningitis. Isolates obtained from 21 patients during 2000–2013 were investigated by phenotypic tests, multilocus sequence typing (MLST), analysis of the TR9 locus from the multilocus variable number tandem repeat (VNTR) analysis (MLVA) scheme and pulsed-field gel electrophoresis (PFGE) of SmaI-digested DNA. Determinants of virulence and antimicrobial resistance were detected by polymerase chain reaction (PCR) and analysed by sequencing. All isolates represented sequence type 1 (ST1) and were suggested to be serotype 2. PFGE and analysis of the TR9 locus allowed the discrimination of four and 17 types, respectively. Most of the isolates were haemolysis- and DNase-positive, and around half of them formed biofilm. Genes encoding suilysin, extracellular protein factor, fibronectin-binding protein, muramidase-released protein, surface antigen one, enolase, serum opacity factor and pili were ubiquitous in the studied group, while none of the isolates carried sequences characteristic for the 89K pathogenicity island. All isolates were susceptible to penicillin, cefotaxime, imipenem, moxifloxacin, chloramphenicol, rifampicin, gentamicin, linezolid, vancomycin and daptomycin. Five isolates (24 %) were concomitantly non-susceptible to erythromycin, clindamycin and tetracycline, and harboured the tet(O) and erm(B) genes; for one isolate, lsa(E) and lnu(B) were additionally detected. Streptococcus suis isolated in Poland from human invasive infections belongs to a globally distributed clonal complex of this pathogen, enriched in virulence markers. This is the first report of the lsa(E) and lnu(B) resistance genes in S. suis. The online version of this article (doi:10.1007/s10096-016-2616-x) contains supplementary material, which is available to authorized users.