Barcoding deep-water chondrichthyans from mainland Portugal
Barcoding deep-water chondrichthyans from mainland Portugal
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DOI：
10.1071/mf14095
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发表时间：
2015-01-01
影响因子：
1.8
通讯作者：
Figueiredo, I.
中科院分区：
文献类型：
--
作者：
Moura, T.;Silva, M. C.;Figueiredo, I.
大多数深海软骨鱼类分布在世界各地。有许多密切相关的物种具有相似的形态，缺乏明确的诊断特征，允许物种区分导致了广泛的识别问题。在这里，DNA条形码被用来评估细胞色素c氧化酶I（COI）的适用性，以确定深水软骨鱼类，并进一步促进目前正在使用的全球分子数据库。样品由来自嵌合体科、Centrophoridae、Somniosidae、Etmopteridae、Oxynotidae、Dalatiidae、Scyliorhinidae、Mitsukurinidae、Chlamydoselachidae和Hexanchidae的40个个体组成。COI被证明是足够的物种鉴定，几乎所有的推定物种恢复以及支持单系分支，低种内变异。序列匹配对BOLD数据库提供的证据存在的识别问题，特别是在属Hydrolagus，Galeus和Centrophorus。目前的研究结果表明，三个不同的Centrophorus物种发生在葡萄牙大陆，C。squamosus、C. granulosus和C. uyato。样本还包括一个白化病Deania calcea和一个C.鳞状标本无真皮致密物。这项研究更新了目前的知识，深水软骨鱼类的多样性和分布发生沿着葡萄牙海岸，并提供了一个重要的工具，物种识别和一致性的分类命名世界各地。
Most deep-water chondrichthyans occurring off mainland Portugal are distributed worldwide. There are many closely related species with similar morphology, and the lack of diagnostic characters that unambiguously allow species discrimination has led to extensive identification problems. Here, DNA barcodes were used to evaluate the suitability of cytochrome c oxidase I (COI) to identify deep-water chondrichthyans and to further contribute to the worldwide molecular databases currently in use. The sample was composed of 40 individuals from the families Chimaeridae, Centrophoridae, Somniosidae, Etmopteridae, Oxynotidae, Dalatiidae, Scyliorhinidae, Mitsukurinidae, Chlamydoselachidae and Hexanchidae. COI proved to be adequate for species identification, with almost all putative species recovered as well supported monophyletic clades, with low intraspecific variation. Sequence matches against the BOLD database provided evidence for the existence of identification problems particularly within the genera Hydrolagus, Galeus and Centrophorus. Present results suggest that three different Centrophorus species occur off the Portuguese mainland, C. squamosus, C. granulosus and C. uyato. The sample also included an albino Deania calcea and a C. squamosus specimen without dermal denticles. This study updates the current knowledge on diversity and distribution of deep-water chondrichthyans occurring along the Portuguese coast, and provides an essential tool for species identification and consistency of taxonomic designations worldwide.