Expression of tissue inhibitor of metalloproteinases (TIMPs) during early cardiac development
Expression of tissue inhibitor of metalloproteinases (TIMPs) during early cardiac development
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DOI：
10.1016/s0925-4773(02)00016-3
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发表时间：
2002-05-01
影响因子：
2.6
通讯作者：
Cai, DH
中科院分区：
文献类型：
--
作者：
Brauer, PR;Cai, DH
基质金属蛋白酶（Matrix metalloproteinases，MMPs）介导细胞迁移和组织重塑，在心脏发育中起重要作用。我们检测了两种MMP抑制剂TIMP-2和TIMP-3在心脏发育关键阶段的表达模式。TIMP-2和TIMP-3 mRNA在垫细胞形成之前和形成早期的内皮细胞中均有表达。TIMP-2在流出道（OT）和房室（AV）垫细胞内持续表达，而TIMP-3 mRNA在AV垫细胞形成后不久检测不到。随后，TIMP-3 mRNA在OT远端的垫细胞中消失，并且这种损失向心室进展，直到最终所有OT垫细胞都缺乏可检测到的TIMP-3转录物。TIMP-3在重构心肌中也有表达，但TIMP-2无表达。免疫细胞化学证实了这些发现。这些观察结果表明，TIMP-2和TIMP-3在早期心脏发育中具有重要但独特的作用。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Matrix metalloproteinases (MMPs) mediate cell migration and tissue remodeling and a-re important in cardiac development. We examined the expression patterns of two MMP inhibitors, tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3, during critical stages of cardiac development, Both TIMP-2 and TIMP-3 mRNA were expressed in the endocardium prior to and during early cushion cell formation. TIMP-2 was continually expressed within the outflow tract (OT) and atrioventricular (AV) cushion cells at all stages examined, whereas TIMP-3 mRNA was undetectable in the AV cushion cells soon after their formation. Subsequently, TIMP-3 mRNA disappeared in cushion cells of the distal OT and this loss progressed toward the ventricle until eventually all of the OT cushion cells lacked detectable TIMP-3 transcripts. TIMP-3, but not TIMP-2, was also expressed within remodeling myocardium. Immunocytochemistry confirmed these findings. These observations suggest that TIMP-2 and TIMP-3 have important but unique roles in early cardiac development. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.