Regulation of hair follicle development by exosomes derived from dermal papilla cells
Regulation of hair follicle development by exosomes derived from dermal papilla cells
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真皮乳头细胞来源的外泌体对毛囊发育的调节
DOI：
10.1016/j.bbrc.2018.04.067
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发表时间：
2018-06-02
影响因子：
3.1
通讯作者：
Lu, Zhongfa
中科院分区：
文献类型：
--
作者：
Zhou, Lijuan;Wang, Han;Lu, Zhongfa
背景：毛乳头细胞（DPC）在毛囊（HF）的生长、形成和周期调控中起关键作用。DPC-Exos被认为是通过旁分泌机制调节HF生长的，其中exosomes可能起着关键作用。方法：将DPC-Exos在HF周期的不同阶段连续注射到HF中，并通过组织学和免疫组织化学分析来评估其作用。评价DPC-Exos对外根鞘细胞（ORSC）的增殖、迁移和细胞周期状态的影响。结果：DPC-Exos直径约105 nm，表达肿瘤易感基因101、分化簇（CD）9和CD 63。注射DPC-Exos加速了小鼠中HF生长期和延迟退化期的开始。免疫组织化学分析显示，β-连环蛋白和Shh水平上调的皮肤。结论：DPC-Exos有助于调节HF的生长发育，为治疗脱发提供了一条潜在的途径。(C)2018爱思唯尔公司All rights reserved.
Background: Dermal papilla cells (DPCs) play a critical role in the regulation of hair follicle (HF) growth, formation, and cycling. DPCs are thought to regulate HF growth through a paracrine mechanism, in which exosomes may play a critical role.Methods: DPC-Exos were cutaneously injected into HFs at different HF cycle stages and the effects were evaluated by histological and immunohistochemical analyses. The effects of DPC-Exos on proliferation, migration, and cell cycle status of outer root sheath cells (ORSCs) were evaluated. After treatment of DPC-Exos, changes in mRNA and protein levels of beta-catenin and Sonic hedgehog (Shh) in ORSCs were detected.Results: DPC-Exos were approximately 105 nm in diameter and expressed tumor susceptibility gene 101, cluster of differentiation (CD)9, and CD63. Injection of DPC-Exos accelerated the onset of HF anagen and delayed catagen in mice. Immunohistochemical analyses revealed that beta-catenin and Shh levels were upregulated in the skin. In vitro, DPC-Exo treatment enhanced ORSC proliferation and migration, and stimulated the expression of beta-catenin and Shh.Conclusion: DPC-Exos contribute to the regulation of HF growth and development, and provide a potential avenue for the treatment of hair loss. (C) 2018 Elsevier Inc. All rights reserved.