Combining 2D angiogenesis and 3D osteosarcoma microtissues to improve vascularization
Combining 2D angiogenesis and 3D osteosarcoma microtissues to improve vascularization
复制标题
DOI：
10.1016/j.yexcr.2017.08.035
复制
发表时间：
2017-11-15
影响因子：
3.7
通讯作者：
Idoux-Gillet, Ysia
中科院分区：
文献类型：
--
作者：
Chaddad, Hassan;Kuchler-Bopp, Sabine;Idoux-Gillet, Ysia
现在众所周知，血管生成与肿瘤进展、侵袭性、转移的出现以及对癌症治疗的抵抗有关。在这项研究中，为了更好地模拟体内肿瘤血管生成，我们使用了骨肉瘤细胞 (MG-63) 的 3D 培养物，并将其沉积在单层生长的 2D 内皮细胞 (HUVEC) 上。我们报告说，内皮细胞与肿瘤细胞结合能够形成组织良好的网络，并且与新血管相对应的管状结构浸润肿瘤球体。这些血管呈现出管腔并表达特定标记物如 CD31 和胶原 IV。 2D 内皮细胞和肿瘤细胞 3D 微组织的结合也增加了血管生成因子 VEGF、CXCR4 和 ICAM1 的表达。细胞环境是体外肿瘤血管化发展和更接近体内肿瘤的关键点。
Angiogenesis is now well known for being involved in tumor progression, aggressiveness, emergence of metastases, and also resistance to cancer therapies. In this study, to better mimic tumor angiogenesis encountered in vivo, we used 3D culture of osteosarcoma cells (MG-63) that we deposited on 2D endothelial cells (HUVEC) grown in monolayer. We report that endothelial cells combined with, tumor cells were able to form a well organized network, and that tubule-like structures corresponding to new vessels infiltrate tumor spheroids. These vessels presented a lumen and expressed specific markers as CD31 and collagen IV. The combination of 2D endothelial cells and 3D microtissues of tumor cells also increased expression of angiogenic factors as VEGF, CXCR4 and ICAM1. The cell environment is the key point to develop tumor vascularization in vitro and to be closer to tumor encountered in vivo.