Expression of a novel human gene, Human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression
Expression of a novel human gene, Human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression
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DOI：
10.1158/0008-5472.can-03-3822
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发表时间：
2004-05-15
期刊：
影响因子：
11.2
通讯作者：
Mukai, K
中科院分区：
文献类型：
--
作者：
Oikawa, K;Ohbayashi, T;Mukai, K
在黑腹果蝇中，翅膀分离样（wapl）基因编码一种调节异染色质结构的蛋白质。在这里，我们描述了一种新的人类同源物的wapl（称为人类WAPL; hWAPL）。hWAPL mRNA主要在宫颈癌中表达，在所有其他正常组织和肿瘤组织中弱表达。hWAPL在良性上皮中的表达仅限于基底细胞层，而在异型增生中，它越来越多地出现在更浅的细胞层中，并与异型增生的严重程度显着相关。弥漫性hWAPL表达被发现在所有的浸润性鳞状细胞癌检查。此外，过表达hWAPL的NIH 3 T3细胞在注射到裸鼠中时发展成肿瘤。此外，抑制h WAPL表达的RNA干扰诱导SiHa细胞的细胞死亡。这些结果表明hWAPL与细胞生长有关，hWAPL的表达可能在宫颈癌的发生和肿瘤进展中起重要作用。
In Drosophila melanogaster, the wings apart-like (wapl) gene encodes a protein that regulates heterochromatin structure. Here, we characterize a novel human homologue of wapl (termed human WAPL; hWAPL). The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weak expression in all other normal and tumor tissues examined. hWAPL expression in benign epithelia was confined to the basal cell layers, whereas in dysplasias it increasingly appeared in more superficial cell layers and showed a significant correlation with severity of dysplasia. Diffuse hWAPL expression was found in all invasive squamous cell carcinomas examined. In addition, NIH3T3 cells overexpressing hWAPL developed into tumors on injection into nude mice. Furthermore, repression of h WAPL expression by RNA interference induced cell death in SiHa cells. These results demonstrate that hWAPL is associated with cell growth, and the hWAPL expression may play a significant role in cervical carcinogenesis and tumor progression.