THE SEQUENCE OF A 32-420-BP SEGMENT LOCATED ON THE RIGHT ARM OF CHROMOSOME-II FROM SACCHAROMYCES-CEREVISIAE
THE SEQUENCE OF A 32-420-BP SEGMENT LOCATED ON THE RIGHT ARM OF CHROMOSOME-II FROM SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/yea.320100007
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发表时间：
1994-04-01
期刊：
影响因子：
2.6
通讯作者：
KALLESOE, T
中科院分区：
文献类型：
--
作者：
HOLMSTROM, K;BRANDT, T;KALLESOE, T
推导出酿酒酵母第2染色体32 420 bp片段的序列。序列数据显示19个潜在的新基因，覆盖了83.5%的序列。已经克隆并测序了四个基因：部分RIF1、DPB3、MRP-L27和SNF5。除了这4个基因外，还鉴定出至少100个氨基酸的15个开放阅读框（orf），编码潜在的新基因。其中两个ORF重叠，第三个位于另一个ORF内。ORF YBR2039的推定基因产物与线粒体能量传递系统中涉及的解偶联蛋白群同源。我们建议重新定位编码线粒体蛋白YmL27的MRP-L27基因，因为它之前已经被定位在x染色体上。ORF YBR2020在473个氨基酸区域与酵母基因SEC61有很强的同源性，同源性为31.9%，这表明YBR2020是一个新的基因，编码酵母细胞中参与蛋白质易位的蛋白质。其中6个潜在基因与Fast A分析中预测的先前测序基因没有明显的同源性。该序列已存入EMBL数据库，登记号为X76053。
The sequence of a 32 420 bp segment of Saccharomyces cerevisiae chromosome II has been deduced. The sequence data revealed 19 potential new genes covering 83.5% of the sequence. Four genes had already been cloned and sequenced: part of RIF1, DPB3, MRP-L27 and SNF5. Besides these four genes, 15 open reading frames (ORFs) of at least 100 amino acids encoding potential new genes were identified. Two of these ORFs are overlapping add a third is located within another ORF.The putative gene product of ORF YBR2039 was homologous to the group of uncoupling proteins involved in the mitochondrial energy transfer system. We propose a remapping of the MRP-L27 gene encoding the mitoribosomal protein YmL27 as it previously has been mapped on chromosome X. The ORF YBR2020 has a strong homology with a 31.9% identity in a 473 amino acid region to the yeast gene SEC61, suggesting that YBR2020 is a new gene encoding a protein involved in translocation of proteins in the yeast cell. Six of the potential genes do not exhibit any significant homology to previously sequenced genes as predicted in the Fast A analysis. The sequence has been deposited in the EMBL data library under Accession Number X76053.