Evaluation of biocompatibility of mineral trioxide aggregate with an improved rabbit ear chamber
Evaluation of biocompatibility of mineral trioxide aggregate with an improved rabbit ear chamber
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DOI：
10.1111/j.1365-2842.2004.01397.x
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发表时间：
2005-02-01
影响因子：
2.9
通讯作者：
Matsumoto, K
中科院分区：
文献类型：
--
作者：
Masuda, YM;Wang, X;Matsumoto, K
本研究采用改良的兔耳腔，在体内评价了三氧化矿物聚合物（MTA）对结缔组织血管重建的生物相容性。用12只雄性大白兔制作了20只改良兔耳腔，并在耳腔中加入了一种可将材料导入活体血管组织的孔。10个腔室用于MTA，其余10个腔室用于含氢氧化钙的根管充填器（Sealapex），作为比较。将体积约为1.5 mm（3）的材料插入腔室中，并在应用后立即、1、3、5.5、8、24、48、72 h以及每周一次直至4周使用生物显微镜观察血管组织和材料之间的相互作用。结果显示，MTA和Sealapex均在4周内发生结缔组织血运重建，微循环完全恢复。然而，在MTA处理的样品中未观察到Sealapex周围的沉淀屏障和棕色区域。可以得出结论，MTA具有生物相容性，不会对结缔组织的微循环产生任何不良部位影响。
In the present study, the biocompatibility of mineral trioxide aggregate (MTA) to the revascularization of the connective tissues was evaluated by using the improved rabbit ear chamber, in vivo. Twenty improved rabbit ear chamber was prepared from 12 male albino-rabbit by using a well through which a material could be introduced into the living vascular tissue. Ten chambers were provided for MTA, and the remaining 10 chambers were used for a calcium hydroxide-containing root canal sealer (Sealapex), as a comparator. A volume of about 1.5 mm(3) of the materials were inserted into the chamber and the interaction between the vascular tissue and materials was observed by using a biomicroscope immediately after application, at 1, 3, 5.5, 8, 24, 48, 72 h, and once a week up to 4 weeks. The results revealed that revascularization of connective tissue took place with complete recovery of microcirculation within 4 weeks in both MTA and Sealapex. However, the precipitate-barrier and brown zone around periphery of Sealapex was not observed in MTA treated samples. It can be concluded that MTA is biocompatible and does not produce any adverse site effect on microcirculation of the connective tissue.