Hydrolase activity in the venom of the pupal endoparasitic wasp, Pimpla hypochondriaca
Hydrolase activity in the venom of the pupal endoparasitic wasp, Pimpla hypochondriaca
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DOI：
10.1016/j.cbpc.2005.04.010
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发表时间：
2005-07-01
影响因子：
2.2
通讯作者：
Richards, EH
中科院分区：
文献类型：
--
作者：
Dani, MP;Edwards, JP;Richards, EH
毒液从蛹endoparasitoid，丘疹1 hypocyanaca先前已被证明含有生物活性分子的混合物。目前..检查了下棘单胞菌毒液中水解酶活性的存在。使用API ZYM半定量比色试剂盒一致地检测到六种水解酶。检测到的主要水解酶为：酸性磷酸酶、β-葡萄糖苷酶、酯酶、β-半乳糖苷酶、酯酶脂肪酶和脂肪酶。酸性磷酸酶的显色反应最快，最强烈。使用对硝基苯酚磷酸盐作为底物测定毒液酸性磷酸酶的最适pH和比活性，分别为4.8和0.47 nmol对硝基苯酚/min/mug毒液蛋白。氟化钠（IC 50 4.2 x 10（-4）M）和混合物抑制剂2（CI 2）以剂量依赖性方式抑制酸性磷酸酶活性。先前已经显示出对在体外维持的Lacanobia oleracea血细胞的P hypocalaca毒液显示出有效的细胞毒性活性。通过在加入L之前用Cl 2滴定毒液来研究毒液中酸性磷酸酶对这种细胞毒活性的贡献。甘蓝血细胞结果表明，尽管毒液中的酸性磷酸酶活性水平相对较高，但毒液酸性磷酸酶在下棘腹蛛毒液的体外抗血细胞活性中不起作用。皇冠版权所有（c）2005由爱思唯尔公司出版。All rights reserved.
Venom from the pupal endoparasitoid, Pimpla 1 hypochondriaca has previously been shown to contain a mixture of biologically active molecules. Currently.. P. hypochondriaca venom was examined for the presence of hydrolase activity. Six hydrolases were consistently detected using the API ZYM semiquantitative colourimetric kit. The main hydrolases detected were; acid phosphatase, beta-glucosidase, esterase, beta-galactosidase, esterase lipase, and lipase. The most rapid and intense colour reaction was detected for acid phosphatase. The pH optimum and the specific activity of venom acid phosphatase was determined using p-nitrophenol phosphate as a substrate and were 4.8 and 0.47 nmol p-nitrophenol/min/mu g of venom protein, respectively. The acid phosphatase activity was inhibited in a dose dependent manner by sodium fluoride (IC50 4.2 x 10(-4) M), and by cocktail inhibitor 2 (CI 2). P hypochondriaca venom has previously been shown to display potent cytotoxic activity towards Lacanobia oleracea haemocytes maintained in vitro. The contribution of acid phosphatase in venom to this cytotoxic activity was investigated by titrating venom against Cl 2 prior to the addition of L. oleracea haemocytes. The results suggest that, despite the relatively high levels of acid phosphatase activity in venom, venom acid phosphatase plays no role in the antihaemocytic activity of P. hypochondriaca venom in vitro. Crown Copyright (c) 2005 Published by Elsevier Inc. All rights reserved.