Organelle transformation: shoot first, ask questions later.
Organelle transformation: shoot first, ask questions later.
复制标题
细胞器改造：先射击，后提问。
DOI：
10.1016/0968-0004(90)90299-q
复制
发表时间：
1990
影响因子：
13.8
通讯作者：
Fox,TD
中科院分区：
文献类型：
--
作者：
Butow,RA;Fox,TD
线粒体和叶绿体的转化现在可以通过应用微弹轰击将DNA直接传递到活细胞中的这些细胞器中。最近的实验表明，线粒体和叶绿体基因组的反向遗传研究是可行的。此外，外源基因或新基因现在可以稳定地引入线粒体和叶绿体，使研究涉及细胞器的更广泛的分子遗传问题成为可能。
Transformation of mitochondria and chloroplasts is now possible by the application of microprojectile bombardment for the direct delivery of DNA to these organelles in living cells. Recent experiments have shown the feasibility of reverse genetic studies on mitochondrial and chloroplast genomes. Moreover, foreign or novel genes can now be stably introduced into mitochondria and chloroplasts, allowing the study of a much wider range of molecular genetic problems involving organelles than was previously possible.