Evaluation and optimization of the influence of silver cluster ions on the MALDI-TOF-MS analysis of polystyrene nanoplastic polymers
Evaluation and optimization of the influence of silver cluster ions on the MALDI-TOF-MS analysis of polystyrene nanoplastic polymers
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DOI：
10.1039/d1ay02219a
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发表时间：
2022-02-03
影响因子：
3.1
通讯作者：
Zhang, Xian
中科院分区：
文献类型：
--
作者：
Habumugisha, Theogene;Zhang, Zixing;Zhang, Xian
在使用基质辅助激光解吸/电离飞行时间质谱法分析聚苯乙烯纳米塑料（PSN）（一种非极性聚合物（NP））时，（MALDI-TOF-MS）中，使用银盐作为阳离子化试剂，同时带来了银簇干扰MS的PSN信号的潜在问题。为了检测PSN，将三氟乙酸银（AgTFA）和硝酸银（AgNO 3）与五种极性基质混合，即2-（4-羟基苯基偶氮）苯甲酸（HABA）、地蒽酚（DI）、芥子酸（SA）、反式-3-吲哚丙烯酸（IAA）和2，5-二羟基苯甲酸（DHB），以及三种非极性基质，即芘（PRN）、蒽（ATH）和苊（ACTH）。结果表明，在m/z 1000-4000范围内检测到银盐团簇离子。五种极性基质与银盐产生银簇，其干扰PSN质谱中的信号，但这些基质与氯化铜II（CuCl 2）盐的组合不产生铜相关簇。然而，使用非极性基质如PRN、ATH或ACTH显著降低了银盐簇离子的信号，并且这种基质类型的改变被认为是用于银簇离子的有前途的优化方法。优化了非极性基质条件而不产生银簇离子，并且发现最佳检测条件是在非极性基质（例如，芘）与银盐（例如，AgTFA）。结果表明，当极性基质如HABA、DI、SA、IAA和DHB与银盐在MALDI-TOF-MS分析中组合时，在m/z 1000-4000范围内检测到银相关簇。抑制银簇离子的产生可以通过使用非极性基质（例如，PRN）或极性矩阵（例如，DHB）与铜盐。
In the analysis of polystyrene nanoplastics (PSNs), a nonpolar polymer (NP), using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), silver salts were used as cationization reagents and simultaneously brought the potential problems of silver clusters that interfered with the PSN signal of MS. To detect PSNs, silver trifluoroacetate (AgTFA) and silver nitrate (AgNO3) were mixed with five polar matrices, namely 2-(4-hydroxyphenylazo) benzoic acid (HABA), dithranol (DI), sinapic acid (SA), trans-3-indoleacrylic acid (IAA), and 2,5-dihydroxybenzoic acid (DHB), and three nonpolar matrices, namely pyrene (PRN), anthracene (ATH) and acenaphthene (ACTH). The results showed that silver salt cluster ions were detected in the range of m/z 1000-4000. Five polar matrices with silver salts produced silver clusters, which interfered with the signals in the mass spectrum of PSNs, but the combination of these matrices with copper II chloride (CuCl2) salt did not produce copper-related clusters. However, the use of nonpolar matrices such as PRN, ATH or ACTH significantly decreased the signals of silver salt cluster ions, and this alteration of matrix types is considered a promising optimization approach for silver cluster ions. The nonpolar matrix conditions were optimized without producing silver cluster ions and the optimal detection conditions were found to be under nonpolar matrices (e.g., pyrene) with silver salts (e.g., AgTFA). The results suggest that when polar matrices, such as HABA, DI, SA, IAA, and DHB, are combined with silver salts in MALDI-TOF-MS analysis, silver-related clusters are detected in the range of m/z 1000-4000. Inhibition of the production of silver cluster ions can be achieved by the use of a nonpolar matrix (e.g., PRN) or polar matrix (e.g., DHB) with copper salts.