ANTI-LYMPHOPROLIFERATIVE ACTIVITY OF BROWN ADIPOSE-TISSUE OF HIBERNATING GROUND-SQUIRRELS IS MAINLY CAUSED BY AMP
ANTI-LYMPHOPROLIFERATIVE ACTIVITY OF BROWN ADIPOSE-TISSUE OF HIBERNATING GROUND-SQUIRRELS IS MAINLY CAUSED BY AMP
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DOI：
10.1016/0742-8413(95)00000-3
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发表时间：
1995-09-01
期刊：
影响因子：
--
通讯作者：
VANREGENMORTEL, MHV
中科院分区：
文献类型：
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作者：
ATANASSOV, CL;NAEGELI, HU;VANREGENMORTEL, MHV
从冬眠黄鼠的棕色脂肪中分离出分子量< 1 kDa的组分（黄鼠）经脱脂、酸提、超离心、超滤等方法分离得到的粗提物，对小鼠淋巴结T淋巴细胞的增殖有明显的抑制作用。从春季活动的地松鼠的棕色脂肪中获得的分子量< 1 kDa的级分没有显示出这种活性。生物活性组分的进一步HPLC纯化及其最有效的抗淋巴组织增生组分的化学和结构分析表明，这是腺苷5 '-单磷酸（AMP）。这些数据支持的概念，在冬眠的哺乳动物AMP起源，至少部分地，从棕色脂肪下调季节性依赖性增殖的胸腺。
A fraction with mol wt < 1 kDa was obtained from the brown fat of hibernating ground squirrels (Citellus undulatus) by means of delipidization, acid extraction, ultracentrifugation and ultrafiltration, This fraction suppressed the proliferation of mouse lymph node cells under standard mitogenic stimuli for T lymphocytes, In contrast, the fraction with mol wt < 1 kDa obtained from the brown fat of active ground squirrels in spring did not display such activity. Further HPLC purification of the biologically active fraction and chemical and structural analysis of its most potent antilymphoproliferative component revealed that this is adenosine 5'-monophosphate (AMP). These data lend support to the notion that in hibernating mammals AMP originating, at least partly, from the brown fat down-regulates the seasonally-dependent proliferation of the thymus.