Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium.
Use of a shuttle vector for the transformation of the white rot basidiomycete, Phanerochaete chrysosporium.
复制标题
使用穿梭载体转化白腐担子菌、金孢原毛平革菌。
DOI：
10.1016/0006-291x(89)92659-4
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发表时间：
1989
影响因子：
3.1
通讯作者：
Reddy,CA
中科院分区：
文献类型：
--
作者：
Randall,T;Rao,TR;Reddy,CA
介绍了一种基于穿梭载体的木质素降解丝状真菌黄孢假单胞菌球质体转化系统。该转化载体被命名为pRR12，由酵母整合质粒YIp5、推测的黄孢假单胞菌的自主复制序列（ars）和一个2.2 kb的Pvu II片段组成，该片段携带来自质粒pNG35的kan r决定因子，该片段赋予了对卡那霉素和相关抗生素G418的抗性。利用pRR12载体将2株不同的黄孢假单胞菌ME446和BKM-F转化为G418抗性菌株。每μg载体DNA获得约20个转化子。转化载体pRR12可以通过大肠杆菌转化从转化子的总DNA中恢复，但频率较低。
A novel shuttle vector based spheroplast transformation system for the lignin degrading filamentous fungus P. chrysosporium is described. The transformation vector, designated pRR12, consists of the yeast integration plasmid YIp5, a putative autonomous replication sequence (ars) of P. chrysosporium, and a 2.2 kb Pvu II fragment carrying kan r determinant from plasmid pNG35, which confers resistance against both kanamycin and the related antibiotic G418. Two different strains of P. chrysosporium (ME446 and BKM-F) were transformed to G418 resistance using vector pRR12. Approximately 20 transformants per μg of vector DNA were obtained. The transforming vector pRR12 could be recovered from the total DNA of transformants by E. coli transformation, albeit at a low frequency.