Identification of genes associated with chlorophyll accumulation in flower petals.
Identification of genes associated with chlorophyll accumulation in flower petals.
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DOI：
10.1371/journal.pone.0113738
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yamamizo C
中科院分区：
文献类型：
--
作者：
Ohmiya A;Hirashima M;Yagi M;Tanase K;Yamamizo C
植物有能力防止叶绿素积累，这将掩盖明亮的花朵颜色，在他们的花瓣。相反，叶子含有大量的叶绿素，因为它是光合作用所必需的。器官特异性叶绿素积累的机制尚不清楚。为了确定决定花瓣中叶绿素含量的因素，我们比较了康乃馨（石竹）的非绿色（红色和白色）花瓣（叶绿素含量很低）、淡绿色花瓣（叶绿素含量低）和叶片（叶绿素含量高）不同阶段中叶绿素代谢相关基因的表达。许多基因编码的叶绿素生物合成酶，特别是镁螯合酶的表达，在非绿色花瓣比在叶片中低。非绿色花瓣也表现出较高的表达参与叶绿素降解的基因，包括绿色基因和脱镁叶绿素酶。这些数据表明，康乃馨花瓣中叶绿素的缺乏可能是由于叶绿素生物合成速率低和降解速率高造成的。通过对拟南芥微阵列数据的分析获得了类似的结果。在香石竹中，大多数与叶绿素生物合成相关的基因在淡绿色花瓣和叶片中的表达水平相似，而叶绿素分解代谢基因在淡绿色花瓣中的表达高于叶片。因此，我们推测非绿色和淡绿色花瓣之间叶绿素含量的差异是由于叶绿素生物合成水平的不同。我们的研究为未来的分子和遗传研究的器官特异性叶绿素积累提供了基础。
Plants have an ability to prevent chlorophyll accumulation, which would mask the bright flower color, in their petals. In contrast, leaves contain substantial amounts of chlorophyll, as it is essential for photosynthesis. The mechanisms of organ-specific chlorophyll accumulation are unknown. To identify factors that determine the chlorophyll content in petals, we compared the expression of genes related to chlorophyll metabolism in different stages of non-green (red and white) petals (very low chlorophyll content), pale-green petals (low chlorophyll content), and leaves (high chlorophyll content) of carnation (Dianthus caryophyllus L.). The expression of many genes encoding chlorophyll biosynthesis enzymes, in particular Mg-chelatase, was lower in non-green petals than in leaves. Non-green petals also showed higher expression of genes involved in chlorophyll degradation, including STAY-GREEN gene and pheophytinase. These data suggest that the absence of chlorophylls in carnation petals may be caused by the low rate of chlorophyll biosynthesis and high rate of degradation. Similar results were obtained by the analysis of Arabidopsis microarray data. In carnation, most genes related to chlorophyll biosynthesis were expressed at similar levels in pale-green petals and leaves, whereas the expression of chlorophyll catabolic genes was higher in pale-green petals than in leaves. Therefore, we hypothesize that the difference in chlorophyll content between non-green and pale-green petals is due to different levels of chlorophyll biosynthesis. Our study provides a basis for future molecular and genetic studies on organ-specific chlorophyll accumulation.
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影响因子：
4.6
作者：
Lysenko, Vladimir;Varduny, Tatyana
通讯作者：
Varduny, Tatyana
影响因子：
7.4
作者：
Barry, Cornelius S.;McQuinn, Ryan P.;Giovannoni, James J.
通讯作者：
Giovannoni, James J.
影响因子：
4.3
作者：
Kruse, E;Grimm, B;Kloppstech, K
通讯作者：
Kloppstech, K
DOI：
10.1073/pnas.96.4.1744
发表时间：
1999-02-16
影响因子：
11.1
作者：
Hansson, A;Kannangara, CG;Hansson, M
通讯作者：
Hansson, M
DOI：
10.1007/978-94-007-1579-0_6
发表时间：
2012-01-01
期刊：
PHOTOSYNTHESIS: PLASTID BIOLOGY, ENERGY CONVERSION AND CARBON ASSIMILATION
影响因子：
--
作者：
Croce, Roberta
通讯作者：
Croce, Roberta