Meisetz and the birth of the KRAB motif
Meisetz and the birth of the KRAB motif
复制标题
DOI：
10.1093/bioinformatics/btl498
复制
发表时间：
2006-12-01
期刊：
影响因子：
5.8
通讯作者：
Ponting, Chris P.
中科院分区：
文献类型：
--
作者：
Birtle, Zoe;Ponting, Chris P.
哺乳动物中最大的转录因子家族是Cys（2）His（2）锌指蛋白，每个锌指蛋白具有NH 2-末端KRAB基序。这个家族的广泛扩展已经发生在不同的哺乳动物谱系中，在人类基因组中已知有近似400个这样的基因。尽管它们广泛存在，但KRAB基序的进化起源尚不清楚，因为以前在四足动物以外的脊椎动物中尚未发现。在这里，我们表明，同源的组蛋白甲基转移酶Meisetz是存在于海胆（球海胆purpuratus）基因组。海胆和哺乳动物Meisetz序列各自含有N-末端KRAB基序，从而在棘皮动物和脊索动物谱系分化之前建立了KRAB基序的早期起源。最后，我们提出的证据表明，KRAB基序来自一个新的家庭KRI（KRAB内部）基序，存在于动物，植物和真菌的最后共同祖先。
The largest family of transcription factors in mammals is of Cys(2)His(2) zinc finger-proteins, each with an NH2-terminal KRAB motif. Extensive expansions of this family have occurred in separate mammalian lineages, with similar to 400 such genes known in the human genome. Despite their widespread occurrence, the evolutionary provenance of the KRAB motif is unclear since previously it has not been found outside of the tetrapod vertebrates. Here, we show that homologues of the histone methyltransferase Meisetz are present within the sea urchin (Strongylocentrotus purpuratus) genome. Sea urchin and mammalian Meisetz sequences each contain an N-terminal KRAB motif, which thereby establishes an early origin of the KRAB motif prior to the divergence of echinoderm and chordate lineages. Finally, we present evidence that KRAB motifs derive from a novel family of KRI (KRAB Interior) motifs that were present in the last common ancestor of animals, plants and fungi.