Drosophila PAT1 is required for Kinesin-1 to transport cargo and to maximize its motility
Drosophila PAT1 is required for Kinesin-1 to transport cargo and to maximize its motility
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DOI：
10.1242/dev.048108
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发表时间：
2010-08-15
期刊：
影响因子：
4.6
通讯作者：
Palacios, Isabel M.
中科院分区：
文献类型：
--
作者：
Loiseau, Philippe;Davies, Tim;Palacios, Isabel M.
Kinesin重链（KHC）是Kinesin-1的发力成分，是果蝇卵母细胞中oskar mRNA定位和细胞核锚定所必需的。这些事件对于前后轴和背腹轴的建立至关重要。KHC对于动力蛋白的定位和所有的卵浆流动也是必不可少的。有趣的是，没有激酶蛋白轻链的卵母细胞在这些依赖KHC的过程中没有显示出主要缺陷，这表明KHC结合其货物并通过一种新的机制被激活。在此，我们对Kinesin在种系中功能的分子机制有了新的认识。结合遗传、生化和运动跟踪研究，我们发现app结合蛋白PAT1与kinein -1相互作用，在oskar mRNA和Dynein的运输中起作用，并且是KHC沿微管有效运动所必需的。这项工作表明，PAT1在细胞内货物运输中的作用与PAT1作为运动蛋白运动的积极调节因子的功能有关。
Kinesin heavy chain (KHC), the force-generating component of Kinesin-1, is required for the localization of oskar mRNA and the anchoring of the nucleus in the Drosophila oocyte. These events are crucial for the establishment of the anterior-posterior and dorsal-ventral axes. KHC is also essential for the localization of Dynein and for all ooplasmic flows. Interestingly, oocytes without Kinesin light chain show no major defects in these KHC-dependent processes, suggesting that KHC binds its cargoes and is activated by a novel mechanism. Here, we shed new light on the molecular mechanism of Kinesin function in the germline. Using a combination of genetic, biochemical and motor-tracking studies, we show that PAT1, an APP-binding protein, interacts with Kinesin-1, functions in the transport of oskar mRNA and Dynein and is required for the efficient motility of KHC along microtubules. This work suggests that the role of PAT1 in cargo transport in the cell is linked to PAT1 function as a positive regulator of Kinesin motility.