DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS INFECTIONS IN MEN AND WOMEN BY TESTING FIRST-VOID URINE BY LIGASE CHAIN-REACTION
DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS INFECTIONS IN MEN AND WOMEN BY TESTING FIRST-VOID URINE BY LIGASE CHAIN-REACTION
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DOI：
10.1128/jcm.32.11.2682-2685.1994
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发表时间：
1994-11-01
影响因子：
9.4
通讯作者：
MAHONY, JB
中科院分区：
文献类型：
--
作者：
CHERNESKY, MA;JANG, D;MAHONY, JB
1993年4月至9月，加拿大汉密尔顿的305名男性和447名女性同意分别收集尿道或宫颈拭子进行培养，20毫升首次空尿（FVU）用于衣原体酶免疫测定和连接酶链反应（LCR）测试，该测试采用雅培实验室的一种试剂盒，称为LCx沙眼衣原体。每个标本的测试性能评估是在扩大的“金标准”的基础上计算的，该“金标准”是通过培养或确认的非培养试验发现阳性的患者。通过使用这一扩大的标准，确定女性的感染率为6%(27/447)，男性的感染率为18.4%（56/305）。两项研究中FVU的LCR检测是最敏感的方法（96%）。衣原体酶的表现如下：宫颈拭子，敏感性78.3%；女性FVU敏感度37%；男性FVU敏感度67.9%。培养是诊断最不敏感的方法：女性宫颈，55.6%；男性尿道，37.5%。男性或女性FVU的LCR检测诊断出最多数量的泌尿生殖道感染，无假阳性。
From April to September 1993, 305 men and 447 women in Hamilton, Canada, consented to the collection of a urethral or cervical swab, respectively, for culture and 20 ml of first-void urine (FVU) for testing by the enzyme immunoassay Chlamydiazyme and by ligase chain reaction (LCR) in the form of a kit from Abbott Laboratories called LCx Chlamydia trachomatis. Evaluation of test performance with each specimen was calculated on the basis of an expanded ''gold standard'' of a patient found to be positive by culture or by a confirmed nonculture test. By using this expanded standard, the prevalence of infection was determined to be 6% (27/447) for the women and 18.4% (56/305) for the men. LCR testing of FVU in both studies was the most sensitive approach (96%). The performance of Chlamydiazyme was as follows: cervical swab, 78.3% sensitivity; female FVU, 37% sensitivity; and male FVU, 67.9% sensitivity. Culture was the least sensitive approach to diagnosis: female cervix, 55.6%; and male urethra, 37.5%. LCR testing of FVU from men or women diagnosed the greatest number of genitourinary tract infections with no false positives.