Electroacupuncture Regulates Disorders of Gut-Brain Interaction by Decreasing Corticotropin-Releasing Factor in a Rat Model of IBS
Electroacupuncture Regulates Disorders of Gut-Brain Interaction by Decreasing Corticotropin-Releasing Factor in a Rat Model of IBS
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DOI：
10.1155/2019/1759842
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发表时间：
2019-10-13
影响因子：
2
通讯作者：
Zhou, Si-yuan
中科院分区：
文献类型：
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作者：
Chen, Ying;Zhao, Yan;Zhou, Si-yuan
客观的。针灸对肠易激综合症（IBS）有效；然而，其作用机制尚未完全了解。我们的目的是探索电针（EA）在肠-脑相互作用障碍的双重调节中的机制。方法。 IBS 大鼠模型是通过慢性不可预测的温和应激 (CUMS) 产生的。 32只大鼠中的8只被分配到空白对照组。其余 24 只大鼠接受 CUMS 治疗 14 天。然后将造模成功的存活大鼠随机分为CUMS组、CUMS+EA组、CUMS+PB(匹维溴铵)组。在接下来的14天治疗中，CUMS+EA组大鼠每天针刺ST25（天枢）、ST36（足三里）、SP6（三阴交）和LR3（太冲）15分钟。 CUMS+PB组大鼠每天灌胃2.7 mg/mL匹维溴铵。在基线、第15天和第30天测量内脏痛阈值、高架十字迷宫测试（EPMT）中张开臂的时间百分比（OT％）以及蔗糖偏好测试（SPT）中的蔗糖偏好（SP％）。 闭合小带-1（ZO-1）的表达、肠上皮结缔结构的形态、CRF和CRF-R1 mRNA的表达实验结束时测量下丘脑、肠粘膜肥大细胞（IMMC）和CRF-R1的双重染色。结果。与空白对照组比较,CUMS组内脏痛阈压、下丘脑ZO-1表达量、OT%、SP%、CRF、CRF-R1 mRNA表达量、IMMC、CRF-R1双染均显着降低。同时，CUMS组结缔结构形态不明显。与CUMS组相比,CUMS+EA组SP%显着升高,而CUMS+PB组无显着性差异。两个治疗组的结缔结构形态清晰可见。并且上述其他参数的表达在两个治疗组中均明显增加。与CUMS+PB组相比,CUMS+EA组ZO-1的表达显着增强。两个治疗组之间其他参数没有发现明显差异。结论。电针治疗可降低下丘脑CRF和CRF-R1的表达，缓解焦虑和抑郁，同时降低胃肠粘膜CRF-R1的表达，增加ZO-1的表达，调整紧密连接（TJs）修复肠粘膜屏障。上述作用表明，EA可能在缓解IBS的胃肠道和心理症状方面发挥双重作用，提示EA在调节IBS大鼠肠-脑相互作用障碍方面具有潜在的双重治疗作用。
Objective. Acupuncture is effective for irritable bowel syndrome (IBS); however, the mechanisms of action are not fully understood. We aim to explore the mechanism of electroacupuncture (EA) in the dual regulation of disorders of gut-brain interaction. Methods. A rat model of IBS was generated by chronic unpredictable mild stress (CUMS). Eight of 32 rats were assigned to the blank control group. The remaining 24 rats received CUMS for 14 days. Then, the rats surviving and successfully modelled were randomly divided into the CUMS group, the CUMS+EA group, and the CUMS+PB (pinaverium bromide) group. In the next 14 days of treatment, rats in the CUMS+EA group were acupunctured at ST25 (Tianshu), ST36 (Zusanli), SP6 (Sanyinjiao), and LR3 (Taichong) for 15 min every day. Rats in the CUMS+PB group were treated by the administration of gavage with 2.7 mg/mL pinaverium every day. Visceral pain threshold, the percentage of time spent in open arms (OT%) in the elevated plus maze test (EPMT), and the sucrose preference (SP%) in the sucrose preference test (SPT) were measured at baseline, day 15, and day 30. The expression of zonula occludens-1 (ZO-1), the morphology of the connective structure of intestinal epithelium, the CRF and CRF-R1 mRNA expression in the hypothalamus, and the double staining of intestinal mucosal mast cells (IMMC) and CRF-R1 were measured at the end of the experiment. Results. Compared with the blank control group, visceral pain threshold pressure, the expression of ZO-1, OT%, SP%, CRF, and CRF-R1 mRNA expression in the hypothalamus, and double staining of IMMC and CRF-R1 were decreased significantly in the CUMS group. Meanwhile, the morphology of the connective structure in the CUMS group was indistinct. Compared with the CUMS group, SP% was significantly increased in the CUMS+EA group, but there was no significant difference for it in the CUMS+PB group. The morphology of the connective structure in the two treatment groups was clear and seeable. And the expression of other parameters mentioned above was apparently increased in the two treatment groups. Compared with the CUMS+PB group, the expression of ZO-1 in the CUMS+EA group was significantly enhanced. And no obvious difference for other parameters was found between the two treatment groups. Conclusions. EA treatment can decrease the expression of hypothalamic CRF and CRF-R1, relieve anxiety and depression, meanwhile reduce the expression of CRF-R1 in the gastrointestinal mucosa, increase ZO-1 expression, and adjust tight junctions (TJs) to repair the intestinal mucosal barrier. The above roles suggest that EA may play a dual role in alleviating the gastrointestinal and psychological symptoms of IBS, suggesting a potentially dual therapeutic role for EA in regulating disorders of gut-brain interaction in IBS rats.