A dynamic and complex network regulates the heterosis of yield-correlated traits in rapeseed (Brassica napus L.).
A dynamic and complex network regulates the heterosis of yield-correlated traits in rapeseed (Brassica napus L.).
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DOI：
10.1371/journal.pone.0021645
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Meng J
中科院分区：
文献类型：
--
作者：
Shi J;Li R;Zou J;Long Y;Meng J
虽然已经进行了很多研究，但杂种优势的遗传结构仍然不明确。为了揭示甘蓝型油菜杂种优势的遗传结构，以甘蓝型油菜(Brassica napus L.)双单倍体群体的202个品系为材料，通过随机杂交获得了一个重构的F_2群体。两个群体在3种环境下测定了15个产量相关性状，只有种子产量和8个产量相关性状表现出显著的中亲优势，平均为8.7%(分枝数)~31.4%(种子产量)。在15个与产量相关的性状中发现了数百个QTL和上位性互作，涉及众多效应中等、全基因组分布、热点明显的变量座位。QTL存在各种遗传方式(加性，A；部分显性，PD；完全显性，D；超显性，OD)和上位性互作(加性×加性，AA；加性×显性/显性×加性，AD/DA；显性×显性，DD)，上位性，尤其是AA上位性似乎是油菜杂种优势的主要遗传基础。与标记杂合度与中亲优势/杂交表现的低相关性一致，相当大比例的显性和DD上位性效应为负值，表明杂合性并不总是有利于杂种优势/杂交表现。讨论了我们的结果对进化和作物育种的影响。
Although much research has been conducted, the genetic architecture of heterosis remains ambiguous. To unravel the genetic architecture of heterosis, a reconstructed F2 population was produced by random intercross among 202 lines of a double haploid population in rapeseed (Brassica napus L.). Both populations were planted in three environments and 15 yield-correlated traits were measured, and only seed yield and eight yield-correlated traits showed significant mid-parent heterosis, with the mean ranging from 8.7% (branch number) to 31.4% (seed yield). Hundreds of QTL and epistatic interactions were identified for the 15 yield-correlated traits, involving numerous variable loci with moderate effect, genome-wide distribution and obvious hotspots. All kinds of mode-of-inheritance of QTL (additive, A; partial-dominant, PD; full-dominant, D; over-dominant, OD) and epistatic interactions (additive × additive, AA; additive × dominant/dominant × additive, AD/DA; dominant × dominant, DD) were observed and epistasis, especially AA epistasis, seemed to be the major genetic basis of heterosis in rapeseed. Consistent with the low correlation between marker heterozygosity and mid-parent heterosis/hybrid performance, a considerable proportion of dominant and DD epistatic effects were negative, indicating heterozygosity was not always advantageous for heterosis/hybrid performance. The implications of our results on evolution and crop breeding are discussed.
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影响因子：
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作者：
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