ASTROCYTIC CELL CLONES DERIVED FROM ESTABLISHED CULTURES OF 8-DAY POSTNATAL MOUSE CEREBELLA
ASTROCYTIC CELL CLONES DERIVED FROM ESTABLISHED CULTURES OF 8-DAY POSTNATAL MOUSE CEREBELLA
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DOI：
10.1016/0006-8993(84)90377-9
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
PESSAC, B
中科院分区：
文献类型：
--
作者：
ALLIOT, F;PESSAC, B
具有星形胶质细胞特性的克隆永久细胞系是通过体外自发转化（即不添加致癌物或致癌病毒）后8天的小鼠小脑的外植体培养物建立的。细胞系是通过多阶段过程衍生的。 4个月时出现具有多种形态的缓慢增殖的病灶。培养开始后，细胞逐渐富集，具有均匀的外观。这些细胞可以建立成永久细胞系，从中获得许多克隆。其中一些克隆细胞系与抗 GFAP（胶质纤维酸性蛋白）血清结合，因此看起来是星形胶质细胞。根据其形态，可以区分出 3 种不同类型的 GFAP 阳性克隆。 I型和II型细胞的体细胞较小； I型有几个短突起，而II型有2个突起，其中1个很细很长(>200μm)。 III型细胞具有大而平坦的体细胞并且没有突起。这 3 种类型的克隆细胞系由与体内星形胶质细胞结合的单克隆抗体标记。特别是，3 种单克隆抗体（BSP-3、M2 和 M3）仅以独特的模式与 II 型细胞结合。 I 型和 II 型星形胶质细胞是假二倍体，III 型星形胶质细胞是异倍体。这些不同克隆细胞系的特性非常稳定。获得了小鼠小脑星形胶质细胞样细胞的永久建立的克隆培养物，其可能是纤维（I型）或帆状（III型）星形胶质细胞和高尔基体上皮细胞（II型）的体外对应物。
Clonal permanent cell lines with astrocytic properties were established from explant cultures of 8-day postnatal mouse cerebella after in vitro spontaneous transformation, i.e., without the addition of carcinogens or oncogenic viruses. The cell lines were derived in a multistage process. Slowly proliferating foci with several morphologies appeared 4 mo. after initiation of the cultures and became progressively enriched by cells with a homogeneous appearance. These cells could be established into permanent cell lines from which many clones were obtained. Some of these cloned cell lines bound anti-GFAP [glial fibrillary acidic protein] sera and therefore appeared to be astrocytic. According to their morphology, 3 separate types of these GFAP-positive clones could be distinguished. Type I and II cells had small somata; type I had several short processes, while type II had 2 processes, 1 of which was very thin and long (> 200 .mu.m). Type III cells had large flat somata and no processes. The 3 types of clonal cell lines were labeled by monoclonal antibodies which bind to astrocytes in vivo. In particular, 3 monoclonal antibodies (BSP-3, M2 and M3) bound only to type II cells in a distinct pattern. Type I and II astrocytes are pseudodiploid and type III, heteroploid. The properties of these different clonal cell lines are very stable. Permanently established clonal cultures of mouse cerebellum astrocyte-like cells, which might be the in vitro counterparts of fibrous (type I), or velamentous (type III) astrocytes and of Golgi epithelial cells (type II) were obtained.