Ultrasound enhances transforming growth factor β-mediated chondrocyte differentiation of human mesenchymal stem cells
Ultrasound enhances transforming growth factor β-mediated chondrocyte differentiation of human mesenchymal stem cells
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DOI：
10.1089/1076327041348437
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Watanabe, H
中科院分区：
文献类型：
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作者：
Ebisawa, K;Hata, KI;Watanabe, H
在临床研究和动物模型中，低强度超声（US）可促进骨折修复并增加机械强度。 US 还通过增加软骨细胞的糖胺聚糖合成来促进软骨愈合。由于间充质干细胞（MSC）具有分化为软骨细胞的能力，超声可以促进其分化。在这里，我们评估了超声对 MSC 向软骨细胞分化和软骨基质形成的影响。当用转化生长因子β（TGF-β，10 ng/mL）处理在颗粒中培养的人MSC时，根据阿新蓝染色和聚集蛋白聚糖免疫染色的评估，它们分化为软骨细胞，但未经处理的细胞颗粒则没有。此外，当每天对 TGF-β 处理的细胞沉淀应用 20 分钟低强度超声时，软骨细胞分化得到增强。从生化角度来看，单独使用 TGF-β、同时使用 TGF-β 和 US 处理后，聚集蛋白聚糖沉积分别增加了 2.9 倍和 8.7 倍。相反，细胞增殖和总蛋白量似乎不受这些处理的影响。这些结果表明，低强度超声增强了颗粒培养物中 TGF-β 介导的 MSC 的软骨细胞分化，并且超声的应用可以促进更大的软骨细胞制备和成熟软骨组织的形成。
In clinical studies and animal models, low-intensity ultrasound ( US) promotes fracture repair and increases mechanical strength. US also promotes cartilage healing by increasing glycosaminoglycan synthesis of chondrocytes. As mesenchymal stem cells (MSCs) have the ability to differentiate into chondrocytes, US may promote their differentiation. Here, we evaluated the effects of US on the differentiation of MSCs toward chondrocytes and cartilage matrix formation. When human MSCs cultured in pellets were treated with transforming growth factor beta(TGF-beta, 10 ng/mL), they differentiated into chondrocytes as assessed by alcian blue staining and immunostaining for aggrecan, but nontreated cell pellets did not. Furthermore, when low-intensity US was applied for 20 min every day to the TGF-beta-treated cell pellets, chondrocyte differentiation was enhanced. Biochemically, aggrecan deposition was increased by 2.9- and 8.7-fold by treatment with TGF-beta alone, and with both TGF-beta and US, respectively. In contrast, cell proliferation and total protein amount appeared unaffected by these treatments. These results indicate that low-intensity US enhances TGF-beta-mediated chondrocyte differentiation of MSCs in pellet culture and that application of US may facilitate larger preparations of chondrocytes and the formation of mature cartilage tissue.