Ozone Inhibits APP/Aβ Production and Improves Cognition in an APP/PS1 Transgenic Mouse Model
Ozone Inhibits APP/Aβ Production and Improves Cognition in an APP/PS1 Transgenic Mouse Model
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臭氧抑制 APP/A beta 产生并改善 APP/PS1 转基因小鼠模型的认知
DOI：
10.1016/j.neuroscience.2019.07.027
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发表时间：
2019-10-15
期刊：
影响因子：
3.3
通讯作者：
Williams, John P.
中科院分区：
文献类型：
--
作者：
Lin, Si-Yu;Ma, Jun;Williams, John P.
阿尔茨海默病（Alzheimer's disease，AD）是一种进行性神经退行性疾病，目前尚无有效的治疗方法。越来越多的证据表明，淀粉样β肽（A β）的产生和沉积在这种疾病的病理机制。在我们的研究中，我们研究了臭氧腹腔注射对APP/PS1转基因小鼠模型AD病理学的影响。雄性小鼠（5月龄）每天接受臭氧腹膜内注射（30 μ g/ml或50 μ g/ml）或腹腔穿刺给药，持续25天，并在Morris水迷宫和旷场试验中评价它们在空间学习记忆和工作记忆以及焦虑方面的改善。前额叶皮层和海马淀粉样β前体蛋白（APP），沿着其他相关的生物标志物AD，通过ELISA，蛋白质印迹和免疫组织化学进行测量。结果表明，臭氧改善APP/PS1转基因小鼠的行为和病理恶化，并降低APP的水平，这支持了臭氧在APP/PS1小鼠中的治疗潜力。(C)2019由Elsevier Ltd代表IBRO发布。
Alzheimer's disease (AD) is a progressive neurodegenerative disorder without effective treatment. Accumulating evidence demonstrates the production and deposition of amyloid-beta peptides (A beta) in the pathological mechanism of this disease. In our study, we investigated the effect of an ozone intraperitoneal injection on AD pathology in APP/PS1 transgenic mouse model. The male mice (5-months-old) received either ozone intraperitoneal injection (at 30 mu g/ml or 50 mu g/ml) or abdominocentesis administration daily for 25 days, and they were evaluated in the Morris water maze and the open field test for improvements in spatial learning-memory and working memory and anxious. Prefrontal cortex and hippocampus amyloid-beta precursor protein (APP), along with other relevant biomarkers for AD, were measured through ELISA, western blot and immunohistochemistry. Results showed that ozone ameliorated the behavioral and pathological deterioration of APP/PS1 transgenic mice, and reduced the level of APP, which supports the therapeutic potential of administration of ozone in APP/PS1 mice. (C) 2019 Published by Elsevier Ltd on behalf of IBRO.