A highly selective Hsp90 affinity chromatography resin with a cleavable linker.
A highly selective Hsp90 affinity chromatography resin with a cleavable linker.
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DOI：
10.1016/j.bmc.2012.03.043
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发表时间：
2012-05-15
影响因子：
3.5
通讯作者：
Haystead, Timothy A. J.
中科院分区：
文献类型：
--
作者：
Hughes, Philip F.;Barrott, Jared J.;Carlson, David A.;Loiselle, David R.;Speer, Brittany L.;Bodoor, Khaldon;Rund, Lauretta A.;Haystead, Timothy A. J.
Over 200 proteins have been identified that interact with the protein chaperone Hsp90, a recognized therapeutic target thought to participate in non-oncogene addiction in a variety of human cancers. However, defining Hsp90 clients is challenging because interactions between Hsp90 and its physiologically relevant targets involve low affinity binding and are thought to be transient. Using a chemo-proteomic strategy, we have developed a novel orthogonally cleavable Hsp90 affinity resin that allows purification of the native protein and is quite selective for Hsp90 over its immediate family members, GRP94 and TRAP 1. We show that the resin can be used under low stringency conditions for the rapid, unambiguous capture of native Hsp90 in complex with a native client. We also show that the choice of linker used to tether the ligand to the insoluble support can have a dramatic effect on the selectivity of the affinity media.
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影响因子：
16
作者：
Mollapour, Mehdi;Tsutsumi, Shinji;Donnelly, Alison C.;Beebe, Kristin;Tokita, Mari J.;Lee, Min-Jung;Lee, Sunmin;Morra, Giulia;Bourboulia, Dimitra;Scroggins, Bradley T.;Colombo, Giorgio;Blagg, Brian S.;Panaretou, Barry;Stetler-Stevenson, William G.;Trepel, Jane B.;Piper, Peter W.;Prodromou, Chrisostomos;Pearl, Laurence H.;Neckers, Len
通讯作者：
Neckers, Len
影响因子：
30.8
作者：
Janecke, AR;Thompson, DA;Gal, A
通讯作者：
Gal, A
影响因子：
16
作者：
Mollapour M;Tsutsumi S;Truman AW;Xu W;Vaughan CK;Beebe K;Konstantinova A;Vourganti S;Panaretou B;Piper PW;Trepel JB;Prodromou C;Pearl LH;Neckers L
通讯作者：
Neckers L
影响因子：
5.6
作者：
Immormino, Robert M.;Metzger, Louis E.;Reardon, Patrick N.;Dollins, D. Eric;Blagg, Brian S. J.;Gewirth, Daniel T.
通讯作者：
Gewirth, Daniel T.
影响因子：
16.8
作者：
Hessling, Martin;Richter, Klaus;Buchner, Johannes
通讯作者：
Buchner, Johannes