Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions.
Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions.
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DOI：
10.3390/cells10071723
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发表时间：
2021-07-08
期刊：
影响因子：
6
通讯作者：
DeLouise LA
中科院分区：
文献类型：
--
作者：
Piraino LR;Benoit DSW;DeLouise LA
唾液腺再生对于开发治疗辐射引起的口干症、干燥综合征和其他导致口干的疾病的方法非常重要。采用组织工程策略的培养条件已用于概括腺体结构和功能，以研究和再生唾液腺。本综述的目的是强调该领域的当前趋势，重点是已被证明可以改善体外分泌功能的可溶性因子。我们进行了 PubMed 检索，以确定过去 10 年发表的文章，并对文章进行评估，以确定最有前途的方法和进一步研究的领域。结果显示，细胞外基质模拟物的使用越来越多，例如 Matrigel®、胶原蛋白和各种功能化聚合物。提供支持信号的可溶性因子，包括成纤维细胞生长因子 (FGF) 和神经营养因子，以及 Rho 相关激酶 (ROCK)、表皮生长因子受体 (EGFR) 和转化生长因子 β 受体 (TGFβR) 的化学抑制剂，已显示出包括水通道蛋白 5 (Aqp5) 在内的重要标记物的增加；肌肉、肠道和胃表达 1 (Mist1)；和角蛋白 (K5)。然而，在体内水平上再现组织功能仍然难以捉摸。重点关注组合测试的可溶性因子、细胞和/或基质线索的鉴定可能会进一步增强体外唾液腺分泌功能的维持。这些方法也可能适合体内翻译，以支持功能障碍腺体的成功再生。
Salivary gland regeneration is important for developing treatments for radiation-induced xerostomia, Sjögren’s syndrome, and other conditions that cause dry mouth. Culture conditions adopted from tissue engineering strategies have been used to recapitulate gland structure and function to study and regenerate the salivary glands. The purpose of this review is to highlight current trends in the field, with an emphasis on soluble factors that have been shown to improve secretory function in vitro. A PubMed search was conducted to identify articles published in the last 10 years and articles were evaluated to identify the most promising approaches and areas for further research. Results showed increasing use of extracellular matrix mimetics, such as Matrigel®, collagen, and a variety of functionalized polymers. Soluble factors that provide supportive cues, including fibroblast growth factors (FGFs) and neurotrophic factors, as well as chemical inhibitors of Rho-associated kinase (ROCK), epidermal growth factor receptor (EGFR), and transforming growth factor β receptor (TGFβR) have shown increases in important markers including aquaporin 5 (Aqp5); muscle, intestine, and stomach expression 1 (Mist1); and keratin (K5). However, recapitulation of tissue function at in vivo levels is still elusive. A focus on identification of soluble factors, cells, and/or matrix cues tested in combination may further increase the maintenance of salivary gland secretory function in vitro. These approaches may also be amenable for translation in vivo to support successful regeneration of dysfunctional glands.
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影响因子：
6.1
作者：
Chramiec A;Teles D;Yeager K;Marturano-Kruik A;Pak J;Chen T;Hao L;Wang M;Lock R;Tavakol DN;Lee MB;Kim J;Ronaldson-Bouchard K;Vunjak-Novakovic G
通讯作者：
Vunjak-Novakovic G
DOI：
10.1038/labinvest.2010.5
发表时间：
2010-04
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
通讯作者：
--
影响因子：
23.9
作者：
Czerniecki SM;Cruz NM;Harder JL;Menon R;Annis J;Otto EA;Gulieva RE;Islas LV;Kim YK;Tran LM;Martins TJ;Pippin JW;Fu H;Kretzler M;Shankland SJ;Himmelfarb J;Moon RT;Paragas N;Freedman BS
通讯作者：
Freedman BS
影响因子：
2.5
作者：
Daley, William P.;Kohn, Joshua M.;Larsen, Melinda
通讯作者：
Larsen, Melinda
影响因子：
4.6
作者：
Daley, William P.;Gervais, Elise M.;Larsen, Melinda
通讯作者：
Larsen, Melinda