Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR
Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR
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DOI：
10.1111/j.1600-0765.2009.01250.x
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发表时间：
2010-06-01
影响因子：
3.5
通讯作者：
Takahashi, N.
中科院分区：
文献类型：
--
作者：
Abiko, Y.;Sato, T.;Takahashi, N.
背景与目的：牙周袋内龈下菌斑生物膜菌群的质和量的变化被认为与牙周炎的发生、发展有关。本研究的目的是定量的9种牙周炎相关的细菌和4种链球菌在龈下菌斑中的比例，并评估其与牙周炎quantitatively.Material和Methods：龈下菌斑样本从12个牙周健康的科目，从28例牙周炎。结果：牙周炎患者牙周组织中厌氧菌总数明显高于牙周健康者（p < 0.05），牙周炎患者牙周组织中厌氧菌总数明显高于牙周健康者（p < 0.05）。在专性厌氧菌中，在牙周炎受试者中检测到高比例的坦纳氏菌（2.04 +/-5.27%，p < 0.05）、牙龈卟啉单胞菌（0.54 +/- 1.41%）和隐真杆菌（0.30 +/- 0.96%），但在牙周健康受试者中未检测到。牙周炎组链球菌总数比例明显低于对照组（P < 0.05）。特别是T. P. gingivalis或E.结论：牙周炎患者中，包括隐球菌在内的专性厌氧菌的检出率明显升高（>= 2.78%），链球菌的检出率明显降低（>= 2.78%）。forthysia、牙龈卟啉单胞菌和E.隐球菌主要在牙周炎受试者的微生物群落中鉴定，而链球菌主要在牙周健康受试者的微生物群落中鉴定，表明龈下环境的变化导致更适合专性厌氧菌生存的条件。这些专性厌氧菌在牙周炎患者龈下菌斑中的比例似乎与人类牙周炎的状况有关。
Background and Objective:Qualitative and quantitative changes of the subgingival plaque biofilm microflora in periodontal pockets are thought to be associated with the development and progression of periodontitis. The aims of the present study were to quantify the proportions of nine periodontitis-associated bacterial species and four Streptococcus species in subgingival plaque, and to evaluate their relationship with periodontitis quantitatively.Material and Methods:Subgingival plaque samples were obtained from 12 periodontally healthy subjects and from 28 patients with periodontitis. The amounts of total and target bacteria were measured by quantitative real-time PCR using universal and species-specific primers, respectively.Results:The proportion of total obligate anaerobes was found to be higher in subjects with periodontitis than in periodontally healthy subjects (p < 0.05). Among obligate anaerobes, Tannerella forsythia (2.04 +/- 5.27%, p < 0.05), Porphyromonas gingivalis (0.54 +/- 1.41%) and Eubacterium saphenum (0.30 +/- 0.96%) were detected at high proportions in subjects with periodontitis, but not in periodontally healthy subjects. By contrast, the proportion of total streptococci was lower in subjects with periodontitis (p < 0.05). Specifically, the proportion of T. forsythia, P. gingivalis or E. saphenum increased (>= 2.78%) and the proportion of Streptococcus species decreased to virtually undetectable levels, in subjects with periodontitis.Conclusion:Obligate anaerobes, including T. forthysia, P. gingivalis and E. saphenum, were identified predominantly in microflora from subjects with periodontitis, whereas Streptococcus species were identified predominantly in microflora from periodontally healthy subjects, suggesting a change in the subgingival environment that resulted in conditions more suitable for the survival of obligate anaerobes. The proportion of these obligate anaerobes in the subgingival plaque of subjects with periodontitis appears to be associated with the status of human periodontitis.